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Method of amplifying heme oxidase gene

A heme oxidase and gene technology, which is applied in the fields of molecular biology and clinical medicine, can solve the problems of molecular biology and clinical research limitations, technical difficulties, and high costs, so as to improve the prevention and treatment level, achieve accurate results, and reduce morbidity. Effect

Inactive Publication Date: 2007-09-12
YUNNAN UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, because of the presence of microsatellite polymorphisms in the promoter of the heme oxidase gene, the molecular biology technique used in previous studies - the Genescan method, is technically difficult and expensive, and has been subject to certain restrictions in molecular biology and clinical research. limit
[0004] After literature search, no public report of the same literature as the content of the present invention has been found in China

Method used

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  • Method of amplifying heme oxidase gene

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Embodiment

[0073] Embodiment: (the concrete steps of the present embodiment are described with the summary of the invention part)

[0074] Using the genomic DNA of the HOX-1 promoter as a template, use the degenerate primers of the present invention to amplify the conserved gene sequence of the protease, and then use the Genescan technology to amplify the unknown 5' end gene sequence, put it into the ABI PRISM3770 instrument for polymerization Acrylamide electrophoresis, laser scanning automated DNA sequencing. The sequencing results were analyzed by Genotype software.

[0075] Untitled Hoxl.ST25

[0076] SEQUENCE LISTING

[0077] Yunnan University

[0078] A method for amplifying heme oxidase gene

[0079] 1

[0080] 1

[0081] PatentIn version 3.1

[0082] 1

[0083] 659

[0084] DNA

[0085] Homo sapiens

[0086]

[0087] 5'UTR

[0088] (1)..(541)

[0089]

[0090]

[0091] Intron

[0092] (647)..(659)

[0093]

[...

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Abstract

This invention relates to a gene amplification method of heme oxygenase, belonging to the areas of molecular biology and clinical medicine. This invention chooses HOX-1 coding gene published in GenBank, and uses biological software DNAman for homology analysis to design a pair of degenerate primers according to the conservative sequence. Taking HOX-1 genomic DNA as templates from patients with COPD and healthy smokers, it uses the degenerate primers to amplify the conservative sequence of its coding gene. The HOX-1 protease gene polymorphism amplified by this invention relates to the COPD susceptibility of Han people in southwestern China, and the individual with genotype type HOX-1I (includes L allele) is more susceptible to COPD.

Description

Technical field: [0001] The invention relates to a method for amplifying heme oxygenase (heme oxygenase-1, HOX-1) gene, which belongs to the fields of molecular biology and clinical medicine. Background technique: [0002] COPD is a common respiratory disease, and its morbidity and mortality are increasing year by year. According to research estimates, the prevalence and mortality of COPD will rise to the fourth place in global disease mortality in 2020. However, a method that can effectively prevent and cure COPD has not yet been developed clinically, and the main reason is that the pathogenesis of COPD is still unclear. [0003] In the past ten years, the research on the pathogenesis of COPD mainly focused on protease / antiprotease and oxidase / antioxidase. alpha 1 - Antitrypsin is currently the only research-proven antiproteolytic enzyme associated with COPD disease. In terms of COPD molecular gene research, there have been many studies on oxidation inhibitory enzyme ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12N15/53
Inventor 戴路明张亚平傅炜萍
Owner YUNNAN UNIV
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