Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Erythro micrococcus ZJB-064 and application thereof

A ZJB-064, Rhodococcus rhodochrous technology, applied in the application, bacteria, biochemical equipment and methods, etc., can solve the problems of large consumption of raw materials, pollution, long cycle and so on

Active Publication Date: 2007-09-12
ZHEJIANG UNIV OF TECH
View PDF0 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, as an important intermediate for the synthesis of atenolol, p-hydroxybenzamide also has a good market application prospect. At present, chemical methods are mainly used to synthesize p-hydroxyphenylacetamide at home and abroad. Chemical synthesis usually has a long cycle and processing steps Complexity, large consumption of raw materials, serious pollution and other problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Erythro micrococcus ZJB-064 and application thereof
  • Erythro micrococcus ZJB-064 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Enzyme production medium: glucose 20g / L, yeast extract 5g / L, urea 7.5g / L, sodium glutamate 0.75g / L, K 2 HPO 4 0.5g / L, KH 2 PO 4 0.5g / L, Mg 2 SO 4 0.5g / L, FeSO 4 ·7H 2 O10mg / L, CoCl 2 10mg / L, the balance is deionized water, pH 7.0-7.2. Sterilize at 121°C for 20 minutes, inoculate Rhodococcus erythrococcus ZJB-064 strain, and cultivate for 2 days at pH 6.5-7.5 and temperature 28-32°C.

[0047] The culture solution was centrifuged (12000rpm) to collect the cells, washed twice with pH 7.0, 0.1 mol / L sodium phosphate buffer, then centrifuged to collect the cells of Rhodococcus erythrococcus ZJB-064, and frozen for future use.

[0048] Evenly disperse the frozen bacteria stored in the refrigerator in pH 7.0, 0.1mol / L sodium phosphate buffer solution, and prepare bacteria suspensions with wet bacteria concentrations of about 0.05, 0.1, 0.15, 0.2g / mL .

[0049] Add 18mL of sodium phosphate buffer solution (pH7.0, 0.1mol / L) containing substrate concentration of 2.22...

Embodiment 2

[0052] The preparation of Rhodococcus rubrum ZJB-064 thalline was the same as in Example 1.

[0053] Use HAc-NaAc buffer to prepare solutions with pHs of 3.6, 4.0, 4.4, 4.8, 5.2, and 5.6, and use NaH 2 PO 4 -Na 2 HPO 4 The buffer solution was made into solutions with pHs of 6.2, 6.6, 7.0, 7.4, and 7.8, and Gly-NaOH was used to make solutions with pHs of 8.6, 9.0, 9.4, 9.8, 10.4, and 10.6. The concentrations of the three buffers were all 0.1 mol / L. At the same time, a bacterial suspension with a wet bacterial concentration of about 0.1 g / mL was prepared.

[0054] Add 10mL of buffer solutions with various pH values ​​to the transformation solution, then add 8mL of nitrile aqueous solution with a substrate concentration of 12.5g / L, and then add 2mL of bacterial cell suspension, so that the final concentration of the substrate in the transformation solution is 5g / L. L, the OD value of the conversion solution was determined to be 4.186. As the buffer solution in the conversio...

Embodiment 3

[0057] The preparation of Rhodococcus rubrum ZJB-064 thalline was the same as in Example 1.

[0058] Nitrile buffer solutions containing PHN of 5.555, 11.111 and 22.222g / L were prepared with phosphate buffer (pH7.0, 0.1mol / L), and a bacterial suspension containing 0.1g / mL of bacteria was prepared at the same time. Add 18 mL of prepared nitrile buffer solution to the 50 mL ground-mouth Erlenmeyer flask, and then use a pipette gun to add 2 mL of bacterial suspension to each Erlenmeyer flask at the same time, so that the final concentration of the substrate in the transformation solution is 5 g / L, 10g / L and 20g / L, the final concentration of wet bacteria is 0.01g / mL, and the transformation solution with different substrate concentrations is made in two parallels. Then put it into a water-bath shaker at 30°C for transformation, the shaker speed was 150rpm, 1mL was sampled every 20min, centrifuged immediately, filtered, and the transformation was tracked by HPLC.

[0059] When the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
melting pointaaaaaaaaaa
Login to View More

Abstract

This invention provides ZJB-064(Rhodococcus rubber ZJB-064) of a new microbial strain selected from soil and its application in preparing p-hydroxyben acetamide, in which, said ZJB-064 was preserved in China tipical cultivated subject center in April 14, 2006 with the preservation number of CCTCCNo: M206040 and is used in sybthesizing p-hydroxyben acetamide.

Description

(1) Technical field [0001] The invention relates to a new microbial strain Rhodococcus ruber ZJB-064 (Rhodococcus ruber ZJB-064) screened from soil and its application in preparing p-hydroxyphenylacetamide. (2) Background technology [0002] In 1990, Novo Industri A / S of Denmark reported a hydratase capable of hydrating acyclic, cyclic and heterocyclic nitriles and hydrating nitriles containing acidic or basic groups. The substrates that the enzyme can convert include p-hydroxyphenylacetonitrile, and it is proved that the substitution of the electron-withdrawing group on the benzene ring can promote the hydration of the enzyme, and the substitution of the electron-withdrawing group will inhibit the hydration of the enzyme. But at present, there is no direct report at home and abroad to use p-hydroxyphenylacetonitrile as a substrate to synthesize p-hydroxyphenylacetamide with microorganisms. [0003] p-Hydroxyphenylacetonitrile, the English name is p-hydroxylacetonitrile, re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N15/31C12P13/02
Inventor 郑裕国蔡谦薛亚平柳志强沈寅初
Owner ZHEJIANG UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com