Echinacea purpurea extractive and the preparing method and the content measuring method
A technology of echinacea extract and echinacea, applied in the field of medicine, can solve the problems of inconsistent quality standards of echinacea preparations, loss of polysaccharide content in the extract, different methods, etc., and achieves the effects of high sensitivity, simple steps and low cost
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Embodiment 1
[0033] [Example 1] Preparation and content determination of Echinacea purpurea extract
[0034] Preparation: Take 10kg of Echinacea purpurea, pulverize, add 10 times the amount of 40% ethanol to reflux for extraction for 2 hours, filter, collect the filtrate, and recover the ethanol from the filtrate under reduced pressure to obtain extract (relative density is 1.06); use 10 times the amount of 20% ethanol Extract with ethanol for 1 hour, filter, collect the filtrate, and concentrate under reduced pressure to obtain an extract (relative density is 1.13); combine the obtained extracts, stir evenly, and spray dry to obtain the Echinacea purpurea extract.
[0035] Content determination: Take 50 mg of Echinacea purpurea extract, weigh it accurately, put it in a volumetric flask, add 25 ml of 36:35 methanol-water solution, ultrasonically treat it for 10 minutes, let it cool, add 36:35 methanol-water solution to dilute to the mark, centrifuge for 3 minutes, take the The clear liquid...
Embodiment 2
[0036] [Example 2] Preparation and content determination of Echinacea angustifolia extract
[0037] Preparation: Take 10 kg of Echinacea angustifolia, crush it, add 12 times the amount of 60% ethanol to reflux for extraction for 1 hour, filter, collect the filtrate, and recover the ethanol from the filtrate under reduced pressure to obtain an extract (relative density is 1.07); use 12 times the amount of medicinal dregs extract with water for 1 hour, filter, collect the filtrate, and concentrate under reduced pressure to obtain an extract (relative density is 1.13); combine the obtained extracts, stir evenly, and spray dry to obtain the Echinacea purpurea extract.
[0038] Content determination: Take 50 mg of Echinacea angustifolia extract, weigh it accurately, put it in a volumetric flask, add 35 ml of 36:35 methanol-water solution, ultrasonically treat it for 10 minutes, let it cool, add 36:35 methanol-water solution to dilute to the mark, centrifuge for 3 minutes, Get the s...
Embodiment 3
[0039] [Example 3] Preparation and content determination of Echinacea purpurea extract
[0040]Preparation: Take 10kg of Echinacea chinensis, pulverize, add 15 times the amount of 70% ethanol to reflux for extraction for 3 hours, filter, collect the filtrate, and recover ethanol from the filtrate under reduced pressure to obtain extract (relative density is 1.08); Extract with water for 1 hour, filter, collect the filtrate, concentrate under reduced pressure to obtain the extract (relative density is 1.15); combine the obtained extracts, stir evenly, and spray dry to obtain the extract of Echinacea purpurea.
[0041] Content determination: Take 50 mg of Echinacea purpurea extract, weigh it accurately, put it in a volumetric flask, add 40 ml of 36:35 methanol-water solution, ultrasonically treat it for 10 minutes, let it cool, add 36:35 methanol-water solution to dilute to the mark, centrifuge for 3 minutes, and take The supernatant is used as the test solution; accurately weig...
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