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Helicobacter pylori HP immunoblotting kit

A technology of Helicobacter pylori and immunoblotting, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of time-consuming and labor-intensive, and can not be typed, and achieve the effect of advanced technology, short time and simple operation

Inactive Publication Date: 2007-08-22
SHENZHEN BLOT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These examinations often bring some pain to the patient, and are time-consuming and laborious, and cannot be classified

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Kit composition (based on 40 servings)

[0014] 50ml of concentrated washing solution, 0.5ml of enzyme-linked reagent, 25ml of chromogenic reagent A, 25ml of chromogenic reagent B, 25ml of stop solution, 40 blotting films (in the reaction tank), and standard bands.

[0015] Storage of the kit: It can be stored in a refrigerator at 2-8°C for one year. If it is stored at room temperature (18°C-25°C), it cannot exceed 3 months, and it can be stored for half a year after opening.

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PUM

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Abstract

The invention discloses a immune imprinting regent box of the Helicobacter pylori which includes the condense washing solution, the enzyme linked regent, the color regent A liquid, the color regent B liquid, the stop liquid and the print film in the reacting slot. Firstly, the whole cell protein antigen of the Helicobacter pylori is divided by the SDS-polyacrylamide gel electrophoresis according to the molecular weight, then transfers it into the fibrous membrane nitrate; if the detected blood serum has the antibody, it will disappear the color band in the antigen location by the ELISA. So it can detect the infection type of the Helicobacter pylori according to the molecular weight difference of the masculine band.

Description

technical field [0001] The invention relates to a Helicobacter pylori immunoblotting kit. Background technique [0002] Helicobacter pylori (H. pylori) is an important pathogenic factor of chronic gastritis, peptic ulcer and non-ulcer dyspepsia. Strong pathogenicity, easy to cause stomach diseases; type II, is a low-virulence Helicobacter pylori strain, its toxicity is weak, and there are generally no clinical symptoms after infection. Routine gastroscopic biopsy of the gastric mucosa for histological or bacterial culture examination. These examinations often bring certain pain to the patient, and are time-consuming and laborious, and cannot be classified. The Helicobacter pylori immunoblotting kit we invented is a non-invasive test and can accurately type HP. Contents of the invention [0003] The object of the present invention is to provide a Helicobacter pylori immunoblotting kit, which is based on a large number of domestic and foreign research results, and utilize...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/561G01N33/559
Inventor 马伟民张永顶
Owner SHENZHEN BLOT BIOTECH
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