Organ preserving fluid and its prepn

A technology of organ preservation solution and solution, which is applied in the fields of biology and medicine, can solve the problems of inability to reduce perfusion re-injury, and achieve the effects of preventing capillary leakage, less accumulation in the body, and preventing calcium overload

Inactive Publication Date: 2007-08-22
南京兰卫医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to: aim at the practical problem that the current traditional UW solution cannot reduce the perfusion re-injury in the process of organ transplantation, and provide a method that can prevent calcium overload during organ transplantation preservation and treat ischemia-induced Preservation solution for multi-organ transplantation with obvious protective effect on organ cell damage and preparation method thereof

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of organ preservation solution (take the preparation of 1000ml as an example):

[0032] Preparation of raw materials:

[0033] Hydroxyethyl starch 65g; Potassium lactobionate 41.2g; MgSO 4 ·7H 2 O 1.25g; Adenosine 1.79g; Allopurinol 0.146g; KH 2 PO 4 3.5g; sucrose 10.26g; glutathione 1.02g; diltiazem hydrochloride 20mg; 5mol / L NaOH solution 3ml; enough KOH and double distilled water.

[0034] Among them: the molecular weight of hydroxyethyl starch is 200KD, and the substitution grade is 0.5MS.

[0035] One 1500ml container, three measuring cups, one glass stirring rod.

[0036] making process:

[0037] Specific steps are as follows:

[0038]The ambient temperature is 20-25°C. First prepare 3ml of 5mol / L NaOH solution with deionized water for later use; prepare 20ml of 5mol / L KOH solution for later use; take 5ml of 5mol / L KOH solution, and dissolve 0.146g of allopurinol in it for later use;

[0039] In the configuration, first add 800ml of double di...

Embodiment 2

[0041] Application of organ preservation solution in liver transplantation

[0042] Subjects: domestic pigs

[0043] The domestic pigs were divided into six groups A, B, C, D, E, F, n=12 in each group, in which: n=6 donors, n=6 recipients; the transplanted livers of group A donors were cooled with UW liquid Stored for 12 hours and then transplanted to recipients; donors in group B used the organ preservation solution provided in Example 1 (hereinafter referred to as "the organ preservation solution") for transplantation to recipients after 12 hours of cold storage; donors in group C after transplantation Transplanted livers were stored in UW liquid for 24 hours and then transplanted to recipients; donors in group D were stored in the organ preservation solution for 24 hours and transplanted to recipients; group E donors were stored in UW liquid for 36 hours After transplantation to recipients; transplanted livers from donors in group F were stored in the organ preservation so...

Embodiment 3

[0059] The liver function of the pigs in each group of recipients described in Example 2 was tracked and detected after the liver transplantation operation, and the results are shown in Tables 1 to 6.

[0060] Table 1 A thin pig liver function

[0061] 1 week after surgery

1 month after surgery

2 months postoperative

Three months postoperative

ALT u / L

835±34

731±29

328±50

110±34

ALB g / L

30±2

30±5

32±2

35±3

[0062] Table 2 Pig liver function in group B

[0063] 1 week after surgery

1 month after surgery

2 months postoperative

Three months postoperative

ALT u / L

890±54

710±32

330±44

105±27

ALB g / L

30±5

31±4

31±2

33±3

[0064] Conclusion: There was no significant difference in liver function between groups A and B at each time point.

[0065] Table 3 Pig liver function ...

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Abstract

The present invention relates to one kind of organ preserving fluid and its preparation process. Each 1000 ml of the organ preserving fluid contains 5 mol / L concentration NaOH solution 3 ml, hydroxyethyl starch 58.5-71.5 g, potassium lactobionate 37.08-45.32 g, MgSO4.7H2O 1.12-1.38 g, adenosine 1.61-1.97 g, allopurinol 0.13-0.16 g, KH2PO4 3.15-3.85 g, cane sugar 9.23-11.29 g, glutathione 0.92-1.12 g, diltiazem hydrochloride 20 mg, KOH and double distilled water in proper amount. The organ preserving fluid has pH 7.3-7.5. Its preparation process includes setting double distilled water in 800 ml in some container, adding the said components via stirring, regulating pH with KOH, adding double distilled water to 1000 ml, filtering and abacterial preservation at 0-8 deg.c.

Description

technical field [0001] The invention relates to the fields of biology and medicine, in particular to an organ preservation solution and a preparation method thereof. Background technique [0002] Organ transplantation is a major advance in human medical science in the 20th century. At present, the total number of transplants in the field of liver, kidney, heart, pancreas and bone marrow transplantation in the world has exceeded 700,000 cases (times), and the longest surviving organ transplant recipient has been nearly half a century. Organ transplantation is one of the main directions of medical development in this century, and it is the only effective radical treatment for end-stage organ failure. Any clinical organ transplantation must first have high-quality donors, which is the prerequisite and fundamental guarantee for successful organ transplantation. Organ preservation, one of the three pillars of organ transplantation, is the cornerstone of organ transplantation. ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 孙倍成陆森王尤山罗望岳明
Owner 南京兰卫医学检验所有限公司
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