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Identification of influenza and common cold, and detecting method of influenza antibody

A common cold and influenza technology, applied in the field of disease diagnosis, can solve the problems of difficult control of influenza transmission, difficulty in diagnosis, speculation, etc., and achieve the effect of reducing losses and fighting costs

Inactive Publication Date: 2007-08-15
河南省生物工程技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most epidemic prevention and control units lack the necessary detection methods, making the spread of influenza difficult to control; at the same time, the atypicality of influenza and the mixed infection of multiple pathogens make the disease epidemic complicated and diagnosis more difficult
Since the epidemic cannot be detected and diagnosed in time, immunization is also extremely blind and speculative

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0030] The invention utilizes artificially synthesized MSLLTEVET and MSLLTE (purity not less than 95%) to couple with carrier protein, immunizes mice, prepares and screens monoclonal antibodies, and further completes the development of influenza diagnostic reagents. The specific implementation examples are as follows:

[0031] 1. Obtaining and coupling of polypeptides: the peptides MSLLTEVET and MSLLTE were synthesized by artificial synthesis, and coupled with bovine serum albumin (BSA).

[0032] 2. Preparation of MSLLTEVET and MSLLTE monoclonal antibodies

[0033] The conjugate of MSLLTEVET and MSLLTE was used as immune antigen to immunize BALB / c mice. The spleen lymphocytes of the immunized mice were fused with myeloma cells, and then the monoclonal was obtained by limiting dilution method.

[0034] Specific steps are as follows:

[0035] Take the conjugate of healthy MSLLTEVET and MSLLTE, add Freund's complete adjuvant and dilute to 10mg / ml to immunize mice, 0.1ml / point, ...

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Abstract

This invention belongs to disease dialogue field outside virus and its antigen rapid test method, which relates to flu virus film protein M1 and M2 with N end height conversion MSLLTEVET and its abnormal meter level to process dialogue case through decoration to process flu antigen level test; using MSLLTEVET and its abnormal meter antigen through decoration to process dialogue agent case for flu virus test.

Description

technical field [0001] The present invention belongs to the application of influenza virus antigen epitopes and variant epitopes in rapid diagnosis, in particular to highly conserved antigenic epitopes of the N-terminals of influenza virus membrane proteins M1 protein and M2 protein and antibodies, variant epitopes and the like The invention relates to the application of antibody in the identification of influenza and common cold and the detection of influenza antibody, which belongs to the rapid detection method in vitro in the field of disease diagnosis. Background technique [0002] Influenza and the common cold are two completely different infectious diseases. [0003] The common cold, commonly known as "cold", is characterized by inflammation of the nasopharynx. There are more than 150 viruses that can cause colds, so we have constant colds throughout our lives. Generally speaking, children have about 5 to 8 colds per year on average, and adults have about 2 to 5 cold...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/577
Inventor 李晨阳李玉林陈小科李智涛王云龙
Owner 河南省生物工程技术研究中心
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