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Yeast glucans extraction process

A technology of yeast glucan and extraction process, applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of large waste liquid, high cost, complex process, etc., reduce the amount of acid and alkali, increase added value, and extract The effect of fewer processes

Inactive Publication Date: 2007-08-08
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protein content of acid-base extraction of glucan is low, but the yield is low and the process is complicated, the cost is high, and there is a lot of waste liquid
The ultrasonic extraction of dextran has a higher product yield but a higher protein content.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: A kind of technology that utilizes high temperature and enzyme preparation to combine to extract the glucan in the yeast cell wall, it is realized by following steps:

[0012] (1) High-temperature extraction: use ordinary yeast cell wall as raw material, add water at 1:3 to make a suspension, heat to 105°C for 1 hour, cool down to 40°C, dilute the remaining insoluble matter with water, centrifuge, and wash twice with water.

[0013] (2) Separation: Take the precipitate after centrifugation, dilute with water at 1:15, heat to 30°C, adjust the pH value to 9, add alkaline protease, the amount of which is 0.2% of the precipitate, and enzymatically hydrolyze for 2 hours, neutralize And, the obtained insoluble matter is centrifuged and washed with water.

[0014] (3) Impurity removal: the precipitate washed with water after protease treatment was treated with excess acetone, filtered, and the residue was washed three times with acetone.

[0015] (4) Drying: The...

Embodiment 2

[0017] Embodiment 2: A kind of technology that utilizes high temperature to combine with enzyme preparation to extract the dextran in yeast cell wall, it is realized by following steps:

[0018] (1) High-temperature extraction: use common yeast cell wall as raw material and add water at 1:8 to make a suspension, heat to 150°C, after 6 hours, cool down to 50°C, dilute the remaining insoluble matter with water, centrifuge, and wash 3 times with water.

[0019] (2) Separation: Take the precipitate after centrifugation and dilute it with water at 1:25, heat it to 40°C, adjust the pH value to 11, add papain in an amount of 0.4% of the precipitate, and neutralize after 6 hours of enzymatic hydrolysis. The resulting insoluble matter was centrifuged and washed with water.

[0020] (3) Impurity removal: the precipitate washed with water after protease treatment was treated with excess acetone, filtered, and the residue was washed three times with acetone.

[0021] (4) Drying: The obta...

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PUM

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Abstract

The invention discloses a manufacturing technique of yeast gluglucosan to decompose yeast cellar wall conjugase under high temperature, which compriss the following steps: adopting yeast cell wall as raw material; adding water with weight rate at 1:3-1:8 to make suspension; heating to 105-150 deg.c for 1-6h; cooling; diluting; centrifuging; washing for 2-3 times; removing sediment; adding water with rate at 1;15-1:25; heating to 30-40 deg.c; adjusting pH value to 9-11; stirring; adding proteinase; enzymolyzing 2-6h; neutralizing; centrifuging; washing; disposing through excessive ketone; filtering; washing residual for three times through ketone; freezing sediment at -70 deg.c for 22h; freezing sediment at -60 deg.c for 6-10h; obtaining the product.

Description

Technical field: [0001] The invention relates to a process for extracting yeast glucan by combining high temperature and enzyme preparation, in particular to a process for extracting glucan in yeast cell wall by using high temperature and enzyme preparation. Background technique: [0002] Yeast is an important industrial microorganism. my country is rich in yeast resources. In recent years, the market demand for yeast product diversification has increased, which has accelerated the development of my country's yeast processing industry. However, yeast processing will produce a large amount of yeast cell walls, which contain about 30% β-D glucan. The dextran extracted from the yeast cell wall can activate macrophages, neutrophils, etc., so it can increase the content of leukin, cytokinin and special antibodies, and fully stimulate the body's immune system. At present, the extraction methods of yeast glucan include acid-base method and ultrasonic treatment method. The acid-ba...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/85
Inventor 杜丽平肖冬光祁业明陈叶福郭学武
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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