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A method for assembling RGD on the surface of porous pure titanium dental implant

A technology for dental implants and implants, applied in dentistry, dental implants, dental prosthetics, etc., can solve the problems of inability to maintain the shape of the implants, damage the surface of the implants, etc., and achieve the effect that is beneficial to clinical application.

Inactive Publication Date: 2007-08-01
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with chemical coupling and physical adsorption, RGD is firmly and stably combined with materials, and can form a relatively uniform layer of film with good repeatability, but there are also some disadvantages, for example, the surface of implants used in clinical applications is often specially treated (Sandblasting, acid etching, etc.) are obtained, and it is a surface that can be well combined with bone tissue after a lot of research. It is best not to be damaged during the assembly process of RGD, and the integrity of the implant surface should be kept as much as possible. However, in the chemical coupling process of RGD immobilization, pretreatment is usually carried out first, such as acid and hydrogen peroxide treatment, so that the titanium material has -OH, -COOH, -NH 2 , sulfhydryl, active hydrogen and other groups, and most of these treatment agents used are to destroy the surface of the implant, and cannot maintain the original shape of the implant, which has a certain impact on the clinical application of the implant

Method used

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  • A method for assembling RGD on the surface of porous pure titanium dental implant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Immerse the porous pure titanium dental implant in 0.5mg / ml PEI solution for 90 minutes, take out the implant, and put it in double distilled water for 10 minutes;

[0021] 2) Immerse the implant in NaHCO containing 0.4mg / ml RGD 3 In the solution for 12hrs, take out the implant, wash with double distilled water for 10min;

[0022] 3) Finally, immerse the implant in 0.5mg / 100ml glutaraldehyde solution for 4hrs, take out the implant, and wash with double distilled water for 10min;

[0023] 4) Finally, immerse the implant in 0.4mg / ml RGD NaHCO 3 In the solution for 24hrs, take out the implant, wash with double distilled water for 10min, and dry it for later use;

[0024] 5) Repeat the above steps 1-4 to obtain a multi-layer RGD covering layer (as shown in Figures 3-5).

Embodiment 2

[0026] 1) Immerse the porous pure titanium dental implant in 0.8mg / ml PEI solution for 60 minutes, take out the implant, and put it in double distilled water for 15 minutes;

[0027] 2) Immerse the implant in NaHCO containing 0.7mg / ml RGD 3 In the solution for 9hrs, take out the implant, wash with double distilled water for 15min;

[0028] 3) Finally, immerse the implant in 1mg / 100ml glutaraldehyde solution for 3hrs, take out the implant, and wash with double distilled water for 15min;

[0029] 4) Finally, immerse the implant in 0.7mg / ml RGD NaHCO 3 After 18 hours in the solution, the implant was taken out, washed with double distilled water for 15 minutes, and dried for later use;

[0030] 5) Repeat the above steps 1-4 to obtain a multi-layer RGD covering layer.

Embodiment 3

[0032] 1) Immerse the porous pure titanium dental implant in 1mg / ml PEI solution for 30 minutes, take out the implant, and put it in double distilled water for 20 minutes;

[0033] 2) Immerse the implant in NaHCO containing 1mg / ml RGD 3 After 6 hours in the solution, take out the implant and wash it with double distilled water for 20 minutes;

[0034] 3) Finally, immerse the implant in 1.5mg / 100ml glutaraldehyde solution for 2hrs, take out the implant, and wash with double distilled water for 20min;

[0035] 4) Finally, immerse the implant in 1mg / ml RGD NaHCO 3 After 12 hours in the solution, the implant was taken out, washed with double distilled water for 20 minutes, and dried for later use;

[0036] 5) Repeat the above steps 1-4 to obtain a multi-layer RGD covering layer.

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Abstract

The invention discloses an assembling method of RGD on the porous pure-titanium tooth plant surface, which comprises the following steps: adopting layer-to-layer electrostatic self-assembling technique to make the surface load positive charge through PEI; immersing plant into RGD solution; using attractive elementary of foreign charge to fix RGD on the PEI surface; crossing PEI and RGD through glutaraldehyde; immersing the plant into RGD solution to seal non-connected aldehydo; increasing RGD quantity; repeating these steps to obtain the product.

Description

technical field [0001] The invention relates to assembling RGD on the surface of a dental implant, in particular to a method for assembling RGD on the surface of a porous pure titanium dental implant. Background technique [0002] Immobilizing some biologically active short peptides on the surface of pure titanium or titanium alloy materials can improve the structural characteristics of the implant surface, increase the biological activity of the material surface, strengthen the affinity of cells, and provide bionics for cell adhesion, proliferation, extension and differentiation. Interface to promote implant osseointegration. The RGD (Arg-Gly-Asp) sequence is a biologically active short peptide that is most commonly used on the surface of implants. It is the most common basic structural part of various extracellular matrices and the basic unit of cell recognition systems. There are about 11 integrin-dependent RGD sequences that specifically bind to the extracellular matrix...

Claims

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Application Information

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IPC IPC(8): A61C8/00A61L27/34
Inventor 杨国利何福明赵士芳
Owner ZHEJIANG UNIV
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