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Test paper for semi quantitative detecting oxLDL

A semi-quantitative detection and test strip technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of inability to carry easily, erroneous detection results, and increase costs, and achieves a reduction in economic burden, a high degree of differentiation, and an increase in cost effect

Inactive Publication Date: 2007-07-25
LANZHOU UNIVERSITY
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Problems solved by technology

But all there is obvious defect, wherein, the quantitative detection test paper of colloidal gold and magnetic (fluorescence) nano particle double label needs special instrument, and cost is higher, and can not reach the purpose of carrying convenience; Special instrument, but the monoclonal antibody line with several concentrations on it requires a large amount of monoclonal antibody, which increases the cost. In addition, due to the difference in the user's judgment of the color of the colored marker, it is easy to produce wrong test results

Method used

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  • Test paper for semi quantitative detecting oxLDL

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Embodiment

[0016] Example (1) Acquisition of Hybridoma Cells Secreting Monoclonal Antibodies

[0017] Female BALB / c mice aged 6 to 8 weeks were purchased from the Experimental Animal Center of Lanzhou Institute of Biological Products, license number 2006-007; oxidized low-density lipoprotein was purchased from Beijing Concord Sanyou Technology Development Co., Ltd.

[0018] The process of obtaining hybridoma cells secreting oxLDL monoclonal antibody is as follows:

[0019] 1. Immunization of Mice

[0020] First immunization: Mix the antigen oxLDL with the same amount of complete Freund’s adjuvant and stir well to make the mixture emulsified. The final concentration of the antigen is 50 μg / ml. Take 8-10 weeks old female BALB / c mice and perform four points in the groin and axillary Immunization, each 0.2ml;

[0021] Immunization again: after 3 weeks, the concentration of the antigen oxLDL remained unchanged, and Freund's incomplete adjuvant was used instead, and the immunization was carr...

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Abstract

A test paper used for semiquantitative-detecting oxLDL consists of plastic base plate, sample-loading region formed by micro-porous filtering film, blocking region formed by acetic acid cellulose film, labeling region formed by glass cellulose film, reaction region formed by nitric acid cellulose film and water absorption region. It is featured as setting control zone with antibody and detection zone with anti-mouse IgG on each above said region.

Description

1. Technical field [0001] The invention relates to a test paper for semi-quantitative detection of oxLDL, in particular to a test paper capable of rapid and semi-quantitative detection of oxLDL in serum samples, which can be used in related fields such as clinical medicine, physical fitness monitoring, and home self-examination. 2. Background technology [0002] Oxidized-low-density lipoprotein (oxLDL) is formed by low-density lipoprotein (Low-density lipoprotein, LDL) through three forms of oxidative modification in vivo. Natural LDL contains a large amount of unsaturated fatty acids, which can generate lipid free radicals under the action of oxygen free radicals and other oxidants, mediate the production of more lipid peroxidation, cause a chain reaction, and finally form multiple a reactive aldehyde. These reactive aldehydes combine with the lipid protein apoB in LDL to generate new epitopes to form oxLDL. According to the degree of oxidation, it is divided into slightl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/544
Inventor 李红玉晏磊何丽娟陈瑜
Owner LANZHOU UNIVERSITY
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