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Method for separating and purifying paecilomyces cicadae polysaccharide

A technology for the separation and purification of Paecilomyces cicadae, applied in the field of separation and purification of Paecilomyces cicadae polysaccharides, which can solve the problems of no reports on separation and purification methods

Inactive Publication Date: 2007-07-25
WENZHOU MEDICAL UNIV
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Problems solved by technology

[0003] After retrieval, there is no report on the separation and purification method of Paecilomyces cicadae polysaccharide

Method used

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  • Method for separating and purifying paecilomyces cicadae polysaccharide

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Embodiment 1

[0021] 1. Degreasing: Take Paecilomyces cicada mycelium powder into a 1000ml beaker, add methanol, stir until completely submerged, and degrease at room temperature for 24 hours. The yellow-brown supernatant was recovered, methanol was added to the sediment, and the degreasing was repeated once.

[0022] 2. Water extraction: the degreased Paecilomyces cicadae mycelium powder obtained in step 1 was added to distilled water at a solid-liquid ratio of 1:6 (W / V), and extracted for 2.5 hours at room temperature and at pH=7. Use a 50ml plastic centrifuge tube to centrifuge the mixture at 5000rpm for 15min, evaporate the supernatant on a rotary evaporator at 45°C under reduced pressure, concentrate to 1.5 times the volume of the original drug, then gradually add pre-cooled 4 times the volume of absolute ethanol, and store in a 4°C refrigerator overnight. Collect the precipitate to obtain the crude polysaccharide of Paecilomyces cicadae.

[0023] 3. Deproteinization: Paecilomyces ci...

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Abstract

This invention discloses a method for separating and purifying polysaccharides from Paecilomyces cicadae. The method comprises: extracting polysaccharides from Paecilomyces cicadae by water extraction and alcohol precipitation, purifying by anion exchange column chromatography and gel filtration chromatography, and freeze-drying the extract in vacuum to obtain polysaccharides from Paecilomyces cicadae. The method can retain the bioactivity of natural polysaccharides without influence on the structure, and has low requirement for apparatus and environment. Besides, the method also has such advantages as simple process, and high practicability.

Description

technical field [0001] The invention relates to a method for separating and purifying Paecilomyces cicadae polysaccharides from Paecilomyces cicadae mycelium by applying bioengineering technology. Background technique [0002] Paecilomyces cicadae (Miquel.) Samson] is the conidia stage of the fungus Cordycepscicadae shing in the family Ergotaceae. Body, that is, my country's traditional Chinese medicine cicadae (P.cicadae). Paecilomyces cicadae is closely related to the precious Chinese medicinal material Cordyceps sinensis in origin, that is, it belongs to the same fungus (Cordyceps genus) as Paecilomyces sinensis, the anamorph of Cordyceps sinensis. "Zheng Lei Materia Medica" claims that its nature and flavor are sweet and cold and non-toxic, and "Compendium of Materia Medica" records that cicadas have the same power as cicadas. Paecilomyces cicadae mycelium has sedative, analgesic, anti-radiation, anticonvulsant and antipyretic effects, and Paecilomyces cicadae has littl...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12P19/04
Inventor 金丽琴吕建新
Owner WENZHOU MEDICAL UNIV
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