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New use of plants magnesium chelate zyme H subunit

A chelating enzyme and plant technology, applied in the direction of plant peptides, enzymes, biochemical equipment and methods, etc., can solve problems such as not found

Inactive Publication Date: 2009-10-14
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ABA receptors that regulate seed development, seedling growth, leaf stomatal behavior, and plant stress responses against adversity—the most important ABA receptors have not yet been discovered

Method used

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  • New use of plants magnesium chelate zyme H subunit
  • New use of plants magnesium chelate zyme H subunit
  • New use of plants magnesium chelate zyme H subunit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, the acquisition of transgenic Arabidopsis

[0060] 1. Construction of expression vector

[0061] The transgenic material is Arabidopsis gl1 (ABRC#: CS1644) (Shaw-Jye Wu, Lei Ding, and Jian-Kang Zhu, 1996. SOS1, a Genetic Locus Essential for Salt Tolerance and Potassium Acquisition. The Plant Cell.8: 617- 627) background, Arabidopsis gl1 (ABRC#: CS1644) is a recessive homozygous mutant for the hairless gene of the Arabidopsis ecotype Columbia.

[0062] 1. Construction of CHLH gene overexpression vector pGSA1276-CHLH

[0063]In order to construct mutants that overexpress the CHLH gene (Arabidopsis genomic locus tag (genomic locus tag) is AY070133, BT002311, NM_121366, Z68495 or AY078971), the open read of the gene was first cloned by polymerase chain reaction Frame (ORF), using the double-stranded cDNA obtained by reverse transcription of the total RNA of Arabidopsis gl1 as a template, using the upstream primer (the sequence is 5'-TAGGCGCGCCAAAATGGCTTCGCTT...

Embodiment 2

[0089] Embodiment 2, regulation and control seed germination

[0090] In this experiment, 10 lines were made for T3 generation homozygote of CHLH overexpression strain and T3 generation homozygote of CHLH RNAi strain, and consistent results were obtained. In the following, only the CHLH overexpression line T3 homozygous line 1 and the CHLH RNAi line T3 homozygous line 12 are used as examples to show the results.

[0091] In the germination experiment, Arabidopsis gl1 seeds, T3 homozygous seeds of CHLH overexpression lines, and T3 homozygous seeds of CHLH RNAi strains, 100 seeds were taken for each treatment, three replicates, and the seeds were sown on MS medium (Sigma, Cat. No. #, M5524), add 0, 0.1, 0.5, 1, 3, 5 or 10 μmol / L of 2-cis (+)-ABA (Sigma) into the medium, and place it at 4°C for vernalization for 3 After 48 hours or 60 hours under the light conditions of 20 DEG C, count the germination (taking the appearance of radicle as a standard) and calculate the seed germin...

Embodiment 3

[0092] Embodiment 3, regulation growth

[0093] In this experiment, 10 lines were made for T3 generation homozygote of CHLH overexpression strain and T3 generation homozygote of CHLH RNAi strain, and consistent results were obtained. In the following, only the CHLH overexpression line T3 homozygous line 1 and the CHLH RNAi line T3 homozygous line 12 are used as examples to show the results.

[0094] Arabidopsis gl1 seeds, T3 homozygous seeds of CHLH overexpression lines, T3 homozygous seeds of CHLH RNAi strains, 100 seeds were taken for each treatment, three replicates, stratified and germinated on ordinary MS medium for 48 hours, Then transfer to MS medium containing different concentrations of 2-cis (+)-ABA (0, 0.5, 1, 3, 5 or 10 μmol / L) for vertical culture. Seedling growth was counted after 10 days. The results showed that Arabidopsis gl1 seeds, T3 homozygous seeds of CHLH overexpression lines, and T3 homozygous seeds of CHLH RNAi lines were stratified and germinated on ...

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Abstract

The invention discloses a new application of the plant magnesium chelatase H subunit. The new application is the application of plant magnesium chelatase H subunit in regulating stress resistance of plants, regulating seed germination and regulating plant growth and development. The invention not only has great theoretical significance for plant life science, but also has important application value for crop genetic improvement and agricultural development, especially has important practical value in improving the drought resistance of grain and economic crops.

Description

technical field [0001] The present invention relates to a new application of plant magnesium chelatase H subunit. Background technique [0002] Studies in recent years have shown that magnesium chelatase H subunit (CHLH), as one of the subunits of magnesium chelatase, is involved in the formation of Mg ions into the porphyrin ring-chlorophyll; as a plastid signal molecule, it mediates The expression regulation of photosynthetic metabolism-related nuclear genes. [0003] Abscisic acid (ABA), as an important plant hormone, regulates seed development, leaf stomatal behavior and a series of growth and development processes, and plays a key role in regulating plant resistance to adversity stress. The mechanism of hormone action shows that only after ABA binds to its receptor can it trigger the relevant cell signaling process, start the corresponding rapid cell response, or cause the change of gene expression, and finally lead to the realization of a series of physiological and b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/00C07K14/415C12N15/82
Inventor 张大鹏沈元月王小芳吴赴清杜淑媛曹峥尚轶
Owner CHINA AGRI UNIV
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