Method for preparing functional protein peptide

A functional protein, protease technology, applied in protein food processing, protein food ingredients, food science and other directions, can solve the problems of low hydrolysis degree of cottonseed protein, low enzymatic hydrolysis efficiency, long reaction time, etc., to achieve protein and arginine Effects of high content, low cellulose content, and low gossypol residues

Inactive Publication Date: 2009-09-23
中发麦迪尔生物科技江苏有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing functional protein peptides, which mainly solves the problems of using cottonseed as a suitable raw material for preparing functional protein peptides, reducing gossypol, anti-nutritional factor residues, and improving protein peptide yields; Solve the problems of low hydrolysis degree, long reaction time and low enzymolysis efficiency of cottonseed protein produced by traditional methods

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  • Method for preparing functional protein peptide

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Embodiment Construction

[0024] Examples see figure 1 2. The steps of the method for preparing functional protein peptides:

[0025] Step 1: Using matured dephenolized cottonseed protein as raw material, firstly extract the cottonseed oil from the pretreated cottonseed with 6# solvent oil, remove gossypol with methanol, and then dry and mature the material at 100°C to 120°C for 10 -30 minutes, and then obtained as the raw material of the enzymatic protein peptide.

[0026] Step 2, combined hydrolysis with bromelain and neutral protease. First put the dried material into the enzymolysis reaction tank, stir and soak, first add bromelain, the addition amount is 3000 units / g raw material, after enzymolysis for 1 hour, add A.S1398 neutral protease, the addition amount is 4500 units / gram of raw material for joint hydrolysis. The temperature is 40-50°C, and the reaction time is 2-7 hours. During the hydrolysis process, the pH value on-line control system is used to dynamically control the pH of the reacti...

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Abstract

A method for preparing functional protein peptides. The raw materials are put into an enzymolysis reaction tank, and bromelain and A.S1398 neutral protease are added for joint hydrolysis, and the stirring rate is controlled to decrease in stages. The pH of the material in the enzymolysis reaction tank Carry out dynamic regulation and monitoring to stabilize the pH of the material in the enzymolysis reaction tank at 7.5-8.5, then filter the protein slurry after enzymolysis in a centrifuge, dry it in a spray drying tower, and pack the finished product. The above raw materials are a A mature dephenolized cottonseed protein, the raw material is obtained by the following method: leaching the cottonseed oil from the pretreated cottonseed with 6# solvent oil, removing gossypol with methanol, and then drying the material at a high temperature of 100°C to 120°C Dry-cure for 10-30 minutes. The invention further improves the enzymatic hydrolysis efficiency and the quality of protein peptide products.

Description

(1) Technical field [0001] The invention relates to a method for preparing functional protein peptides, in particular to a method for preparing functional protein peptides by enzymolysis using cottonseed as raw material. (2) Background technology [0002] Protein is a macromolecular substance, which is not easily digested and absorbed by the human body. Therefore, people often use hydrolysis to degrade it into small molecular substances such as peptone, peptide, and amino acid. Not only are they easy to absorb, but short peptides also have some special effects. Cottonseed has a high protein content and is a high-quality protein. However, cottonseed contains gossypol, which is harmful to the human body. The cake after cottonseed oil extraction is often directly processed into feed, and its rich protein has not been well utilized. There are two existing methods of using cottonseed to prepare functional protein peptides. One is to use high-temperature pre-pressed leaching and d...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02A23J3/34
Inventor 焦洋李贤永孙猛白卫华
Owner 中发麦迪尔生物科技江苏有限公司
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