Artificial induction method for a mushroom (Tricholoma matsutake(S. Ito et Imai)Sing)

Abstract

The invention relates to a method for inducing artificial bacteria ponds of matsutake mushrooms, which is characterized in that: a) the container is first washed, rinsed and dried with detergent; Alcohol and 0.05-1.0% sodium hypochlorite solution are used to spray and disinfect the container and the container cover successively, and keep the disinfectant for 5-20 minutes, and the dosage is 5-20ml disinfectant / 100ml container; On the sterilized workbench, let it dry naturally for 30-40 minutes; d) After the container is dried, add the water content to 20% (v / v), add the absolute ethanol (1.4%, v absolute ethanol / v dry soil) ) mixed with olive oil—the oil concentration is 0.5-2.0% (oil v / dry soil v) and the culture soil that has been sterilized by autoclaving is directly put into the container, and then inserted into the matsutake mushroom block or bacterial liquid, and covered or Seal the seal and conduct induction culture at 20-30°C in the dark. The invention successfully induces the artificial fungus pond of the matsutake mushroom, has the advantages of simple and convenient method, is suitable for non-laboratory operation, and is low in cost, and is suitable for practical popularization and application in matsutake mushroom production areas to increase the output of the edible fungus.

Description

technical field [0001] The invention relates to a method for inducing artificial bacteria ponds of matsutake mushrooms, in particular to an experimental method suitable for non-laboratory operations. Background technique [0002] Tricholoma matsutake (S.Ito.et.Imai) Sing.] is one of the most valuable mycorrhizal edible fungi in the world, and it is a national second-class protected plant. In my country, it is mainly distributed in Jilin and Heilongjiang in the northeast, Yunnan and Sichuan in the southwest, and sporadically distributed in other provinces such as Shanxi, Anhui, Taiwan, and Tibet. Foreign countries are mainly distributed in Japan, North Korea, Russia and other countries. Matsutake mushroom is a delicious food rich in protein, multivitamins and a large amount of sugar. In the world, Japanese people like to eat Matsutake mushroom. In recent years, due to the deterioration of the environment and the expansion of demand, Japan's local production has been far from...

AI Technical Summary

Problems solved by technology

However, there are certain defects in these induction methods - such as Yamada (1999b) and Guerin (2003) etc. all use autoclaving to sterilize the induction container, because large-capacity plastic containers or relatively cheap plastic utensils Most of them are not resistant to high temperature, and γ-ray disinfection is a solution to the problem (Tomo Teramura, 2000), but the cost is high, and it is impossible for general laboratories or experimental stations to have such facilities

Therefore, although these induction methods are theoretically feasible, they are very difficult in practice.

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