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Production method for purifying enoxaparin sodium

A technology of enoxaparin sodium and purification method, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems of unreachable, easy to destroy the enoxaparin structure, and the residual pigment cannot be further separated, and achieves easy industrial production. Effect

Inactive Publication Date: 2008-11-26
HANGZHOU JIUYUAN GENE ENG
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  • Abstract
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Problems solved by technology

Since enoxaparin is a hydrophilic polysaccharide, general purification processes such as activated carbon adsorption can remove part of the pigment, but the residual pigment cannot be further separated and cannot meet the standard of European Pharmacopoeia 4; while chemical decolorization method is easy to destroy the color of enoxaparin. structure, thereby affecting the quality of enoxaparin finished products

Method used

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  • Production method for purifying enoxaparin sodium
  • Production method for purifying enoxaparin sodium
  • Production method for purifying enoxaparin sodium

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1, the preparation of enoxaparin sodium

[0032] 1.1 Salification of heparin with long-chain quaternary ammonium salts in aqueous media

[0033] Weigh 100g of heparin raw material and 20g of sodium chloride and dissolve in 1L of water, slowly add 800ml of 95% ethanol at room temperature, after precipitation overnight, remove the supernatant, add 1L of water to dissolve the precipitation. Weigh 250 g of benzylethylammonium and dissolve it in 1 L of water, slowly add it into the heparin solution, and stir. The precipitate was filtered, washed with water, filtered and dried to obtain about 327 g of heparin sodium quaternary ammonium salt.

[0034] 1.2 Benzyl esterified heparin

[0035] The above heparin sodium quaternary ammonium salt was dissolved in 1.2L of dichloromethane, 350ml of benzyl chloride was slowly added, and stirred at 35°C for 25 hours. In addition, 200g of sodium acetate was weighed and dissolved in 2L of methanol, slowly added to the above dich...

Embodiment 2

[0038] Embodiment 2, decolorization method one

[0039] Weigh 23 grams of crude enoxaparin, add water to dissolve, and the colorimetry is equivalent to the yellow No. 3 color specified in European Pharmacopoeia 4. Add activated carbon, stir at 100rpm for 40 minutes, filter with filter paper to remove activated carbon, and then filter with microporous membrane to remove residual activated carbon. Put the enoxaparin sodium solution on a H103 non-polar macroporous polystyrene adsorption resin column (manufactured by Nankai University Chemical Factory), with a column bed volume of 150 ml, and elute with water to collect the target peak. The collected solution was eluted twice on the column to collect the target peak. The colorimetry was better than the yellow No. 5 color specified in European Pharmacopoeia 4, and close to the yellow No. 6 color. The collected solution was precipitated with ethanol, filtered, freeze-dried, and weighed to obtain 18.8 g. After testing, the indicato...

Embodiment 3

[0045] Embodiment 3: decolorization method two

[0046] Weigh 30 grams of enoxaparin crude product, add water to dissolve, and the colorimetry is close to the yellow No. 3 color specified in European Pharmacopoeia 4. Put the enoxaparin solution on an XAD-7HP polar macroporous polyalkylene ester adsorption resin column (manufactured by Rohm Haas), with a column bed volume of 120 ml, and elute with water to collect the target peak. The column was eluted for 2 more times, and the target peak was collected, and the colorimetry was yellow No. 4 color defined in European Pharmacopoeia 4. Add activated carbon, stir at 100rpm for 40 minutes, filter with filter paper to remove activated carbon, and then filter with microporous membrane to remove residual activated carbon. The colorimetry is better than the yellow No. 5 color specified in European Pharmacopoeia 4. The collected solution was precipitated by ethanol, filtered, freeze-dried, and weighed to obtain 26.4 grams. After testin...

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Abstract

The invention relates to a purifying manufacture method for Yino sodium heparin that adopts long chain quaternary ammonium salt salinization heparin, after taking benzyl chloride esterification, gaining the raw Yino heparin raw product. After taking decoloration by active carbon and macropore adsorptive resin, the sodium heparin product could be gained. The invention is easy to realize industrializing producing.

Description

technical field [0001] The invention relates to a method for purifying and producing low molecular weight heparin sodium, in particular to a method for purifying and producing enoxaparin sodium. Background of the invention [0002] The English name of enoxaparin sodium is enoxaparin sodium. According to the definition of European Pharmacopoeia 4, its chemical structure is as follows: [0003] [0004] Among them, n is an integer of 1-21, R is H or SO 3 Na, R' is H or SO 3 Na or CO-CH 3 , when R2 is H, R3 is CO 2 Na or when R2 is CO 2 When Na, R3 is H. [0005] According to the provisions of European Pharmacopoeia 4, enoxaparin sodium belongs to the low molecular weight heparin family. It is obtained from heparin sodium salt extracted from pig intestinal mucosa through benzyl esterification and alkaline depolymerization. The average molecular weight is 4500 The main feature is a 4-enolpyranuronic acid structure at the non-reducing end of the molecule. [0006] The m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/10B01J20/26
Inventor 金飞李辉阮德桂施吉顺陈巍徐飞虎
Owner HANGZHOU JIUYUAN GENE ENG
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