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Application of paris polyphyla var. yunnanensis stem and leaf monomer saponin in pharmaceutical industry

A technology of Dianzhonglou, stem and leaf, applied in the field of Dianzhonglou stem and leaf monomer saponins

Inactive Publication Date: 2008-11-05
云南省肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on the use of aerial parts for medicinal purposes at home and abroad, and there is no research report on the use of monomeric saponins in the stems and leaves of Diana dianthus for anti-leukemia

Method used

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  • Application of paris polyphyla var. yunnanensis stem and leaf monomer saponin in pharmaceutical industry
  • Application of paris polyphyla var. yunnanensis stem and leaf monomer saponin in pharmaceutical industry
  • Application of paris polyphyla var. yunnanensis stem and leaf monomer saponin in pharmaceutical industry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0192] --Preparation of monomeric saponins from stem and leaf of Pachyphyllum dianthus

[0193] Use the stems and leaves of the aboveground part of Dionysus diangi as raw materials, crush them, and extract them with ethanol; after recovering the water-soluble matter after ethanol, put them on the saponin resin column to elute with water, 70% ethanol, and 95% ethanol respectively, and collect the eluted part of 70% ethanol for recovery Ethanol is the total saponins; the total saponins are subjected to silica gel column chromatography and eluted with an organic solvent system (8:2v / v) to obtain ten fractions, and the second, fourth, and sixth and ninth fractions are respectively evaporated to remove the organic solvent to obtain four fractions ; Each fraction is subjected to reverse phase column chromatography, and is eluted with an organic solvent system (8: 2--9: 1v / v) to obtain saponin I, II, III, IV, and individual components; recrystallize respectively, and obtain a purity o...

Embodiment 2

[0195] Effects of Saponins from the Stem and Leaf of Pachyphylla dianthus on the Proliferation of K562 and HL60 Cells

[0196] Saponins I to IV from the stems and leaves of Pachyphyllum yunnanensis can significantly inhibit the proliferation of K562 and HL60 cells, and their effects have a good dose-effect relationship. After calculation, the IC of the stem and leaf saponins I~IV of Pachyphyllum dianthus on K562 cells 50 Respectively 0.81±0.8, 0.08±0.04, 0.45±0.26, 0.18±0.15ug / ml, IC for HL60 50 They were 2.13±1.03, 0.05±0.03, 0.01±0.006, 0.09±0.01ug / ml, all less than 10ug / ml. The results are shown in Table 1, and the concentration-inhibition rate curve is shown in figure 1 , figure 2 .

[0197] The experiment was repeated three times. See Table 1-4 for the inhibitory effects of saponins I to IV from the stems and leaves of Diana polyphylla in K562 cells, and see Table 5 for the effects of VP-16 (positive control group) in inhibiting the proliferation of K562 cells; The...

Embodiment 3

[0229] -- Observation of cell morphology

[0230] After K562 and HL60 cells were treated with 5ug / ml saponin II for 24 hours, the cells were observed under an inverted phase-contrast microscope, and the cells were found to be of different sizes, irregular shapes, and fission and pyknosis. After Hoechst33258 staining, observed under a fluorescent microscope, it was found that the cell volume became smaller, the nucleus was condensed, dense granular strong fluorescence could be seen in the nucleus or cytoplasm, and in severe cases, the cells blew to form apoptotic bodies. Normal cells showed diffuse and uniform fluorescence, and the fluorescence was weaker than that of the treatment group (see image 3 , 4).

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Abstract

The invention relates to a method for using yunnannogenin haulm saponin to produce medicine. Wherein, it uses the haulm of yunnannogenin as material, while yunnannogenin saponin I-IV can restrain the increment of leukemia cell K562 and HL60, while their IC50 value e lower than 10ug / ml; the saponin II can induce K562 and HL60 cell to dry externally, relative to the decrease of Bc1-2 / Bax ratio, which is the molecule target of leukocythemia resistance.

Description

technical field [0001] The present invention relates to the use of monomeric saponin from the stem and leaf of Polygonum dianthus, in particular to the use in the field of pharmacy. Background technique [0002] Leukemia is a malignant tumor that occurs in the hematopoietic system. In my country, the incidence rate of leukemia is 2.76 / 100,000. Among the mortality rate of malignant tumors, leukemia ranks 6th (male) and 8th (female), and ranks 1st among children and adults under 35 years old (Ye Rengao, Lu Zaiying Editor-in-Chief. Internal Medicine. Sixth Edition. Beijing: People's Medical Publishing House, June 2004, 625). In recent years, the research on leukemia has gradually deepened. In the treatment of leukemia, due to the application of effective combined chemotherapy (chemotherapy) and the progress of bone marrow transplantation, many patients have been able to survive without disease for a long time. However, the toxicity of chemotherapy drugs for leukemia is relativ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P35/00C07H17/04
Inventor 何明生陈昌祥王方方单芳
Owner 云南省肿瘤医院
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