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Technique and equipment for crystallizing nucleotide

A nucleotide and crystallization technology, applied in the field of nucleotide purification process and its equipment, can solve the problems of difficult to control the nucleation induction period, high cost of production process, inability to meet demand, etc., and achieve better quality, convenient operation, Good crystal form

Active Publication Date: 2008-10-01
NANJING TECH UNIV
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The equipment requirements of this method are relatively simple, but because it is difficult to control the nucleation induction period in the elution crystallization, a supersaturated solution of NMP with a high degree of supersaturation is usually formed, resulting in the obtained crystalline product often being a powdery precipitate instead of It is not a crystal with large particles, which cannot meet the demand
In addition, a large amount of alcohol is consumed in the crystallization process, and the cost of the production process is relatively high. It takes 180 tons of alcohol to produce 1 ton of AMP crystallization products, and 60 tons of alcohol to produce 1 ton of CMP crystallization products.

Method used

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  • Technique and equipment for crystallizing nucleotide
  • Technique and equipment for crystallizing nucleotide
  • Technique and equipment for crystallizing nucleotide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] combined with figure 1 , firstly, the feed liquid containing only 5'-AMP with a concentration of 6 g / l after separation and initial extraction is sent to the evaporating concentrator 2 with a capacity of 100 L through pipeline 5, and is carried out at a vacuum degree of 695 mm Hg and a temperature of 38.1 ° C. Concentrated in vacuum to 15.2g / l, this supersaturated solution is sent to vacuum suction filter device 9 through insulation pipeline 10, can control the vacuum degree that produces about 700mm Hg by virtue of the effect of vacuum pump 11, after suction filtration, concentrated solution is pumped in feed liquid pump 12 The solution is crystallized by introducing the insulated pipeline into a 500L crystallizer 3 with a constant temperature of 20.5°C and a stirring rate of 100rpm / min. In order to maintain the concentration of the crystallization solution at 12 g / l, which is higher than the solubility at this temperature, the feed rate was controlled at 0.55 L / min. ...

Embodiment 2

[0038] combined with figure 1 , firstly, the feed liquid containing only 5'-CMP with a concentration of 20g / l after the initial extraction of ion exchange is sent to the evaporation concentrator 2 with a capacity of 100L through the pipeline 5, and it is carried out at a vacuum degree of 605mm Hg and a temperature of 45°C. Concentrated in vacuum to 40.2g / l, this supersaturated solution is sent to the vacuum filter device 9 through the insulation pipeline 10, by virtue of the effect of the vacuum pump 11, the vacuum degree of about 700mm Hg can be controlled, and the concentrated solution will be pumped in the feed liquid pump 12 after the suction filtration. The solution is crystallized by introducing the insulated pipeline into a crystallizer 3 with a capacity of 500 L at a constant temperature of 24.8° C. and a stirring rate of 150 rpm / min. In order to maintain the concentration of the crystallization solution at 35.8 g / l, which is higher than the solubility at this temperat...

Embodiment 3

[0042] combined with figure 1 , if in the crystallizer 3, start to add suspension density to be 2.5g / L, particle size is the seed crystal of 0.08mm, concentrate to 16.8g / l in the vacuum evaporation concentrator, crystallize under the situation that crystallization liquid concentration maintains 10g / l , other operating conditions were the same as in Example 1 to complete the continuous crystallization of 5'-AMP. Embodiment 3 can also achieve the purpose of continuous crystallization of 5'-AMP, and the crystallization period is only 21.5 hours. Obviously, the quality of the crystallization product obtained in Example 2 is better than that of the product obtained in Example 1, because the whole crystallization process is based on induced crystallization, and the finished product particles are more uniform, and the average particle size is obviously increased, and the purity and yield are also high. Higher, the results are shown in Table 3, Figure 9 shown.

[0043] AMP finishe...

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Abstract

This invention provides method and device for directly crystallizing nucleoside monophosphate (NMP) from its solution. The process comprises: (1) vaporizing and concentrating crude NMP solution to obtain an oversaturate solution; (2) introducing the oversaturated solution to crystallizer by a heat-insulating pipe, and crystallizing at constant temperature and stirring speed to obtain NMP crystals. The process has such advantages as low cost, high crystal quality, good crystal structure, high crystal purity and high yield.

Description

technical field [0001] The present invention relates to a nucleotide purification process and its equipment, more specifically to the improvement of a nucleotide NMP crystallization process and its equipment. Background technique [0002] At present, according to known nucleotide purification methods, crystals can be obtained by cooling, concentrating, adding seed crystals, adding a hydrophilic solvent that does not dissolve nucleosides, or combining these methods (patent No. JP [31] 181145). [0003] Kiyoshi Nakayama et al. proposed in the literature (patent No. JA17687 / 72): the AMP after separation is first concentrated under reduced pressure, then added ethanol for crystallization to obtain a crude crystal, and then recrystallized to obtain a finished product. This method needs to be carried out Crystallization twice, the cost is higher. [0004] Mi Keyong et al. proposed in the literature (patent number CN1370837) that the enzymatic hydrolysis solution of 5'-nucleotides...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
Inventor 应汉杰吕浩赵谷林
Owner NANJING TECH UNIV
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