Cremastra appendiculata(D.Don)Makino artificial seed preparation method

Abstract

The invention belongs to field of crop seed artificial production technology, and provides a key technology for producing rhododendron orchid which is a medical herb and in shortage. The invention in detail relates to the breeding of original bulb of ghododendron orchid and establishing of floating line of original bulb, preparing the medium material for embedding the artificial seed shell, artificial endosperm and germination medium material, storing the said seed and sowing.

Description

1. Technical field [0001] The invention belongs to the technical field of plant artificial seed production. 2. Background technology [0002] Rhododendron orchid [Cremastra appendiculata (D.Don.) Makino] is a wild rare medicinal and ornamental plant of the orchidaceae Rhododendron orchid, mainly distributed in East Asia, especially in Guizhou and Sichuan of China (ActaPhytotaxonomica Sinica.2003, 41 : 263-266). The pseudobulb is used as medicine, and the medicinal material is called Shiitake mushroom, which is pungent, sweet, cold in nature, and slightly poisonous. Anti-liver cancer, breast cancer, uterine cancer, etc. (Journal of Traditional Chinese Medicine.1996, 16: 243-246; Planta Medica. 2004, 70: 171-173), externally used to treat sores, snake bites, skin burns or Burns, etc. The natural reserves of Rhododendron orchid are extremely scarce, coupled with unplanned artificial excavation, wild resources are on the verge of exhaustion. In order to save this rare species t...

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Problems solved by technology

[0003] However, the cultivation of seeds and seedlings is the key problem that must be solved first for the artificial planting of Chinese medicinal materials. Since rhododendron orchids can hardly reproduce with seeds, the pseudobulb is its only reproductive organ. And each pseudobulb of this plant can only grow in one year. A new pseudobulb is produced, so the propagation speed is extremely slow, which makes it difficult to carry out artificial planting on a large scale. In addition, the normal growth of the plant requires symbiosis with specific fungi, which increases the difficulty of artificial planting. Therefore, to achieve the Artificial planting must first solve the problem of rapid and efficient propagation of its seeds and seedlings. Secondly, it is necessary to isolate and identify the types of symbiotic fungi and cultivate and utilize them.

[0004] Although breakthroughs have been made in the research on tissue culture and rapid propagation of Orchidaceae, there are still considerable difficulties in the rapid propagation of some species of Orchidaceae. The propagation of Rhododendron is an example. In recent years, although We have made great breakthroughs in tissue culture and in vitro rapid propagation of rhododendron orchids, but because of the long production cycle of test tube seedlings, high cost, heavy workload for hardening and transplanting, and inconvenient storage and transportation, there are limitations. Large-scale production. Compared with test-tube seedlings, artificial seeds use less medium, small volume, fast propagation and germination, and convenient transportation and storage. way.

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