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Microbial production of vitamin C

A technology of vitamins and microorganisms, applied in the direction of fermentation, etc., can solve problems such as difficulty in obtaining total output

Inactive Publication Date: 2007-12-26
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method involves many complex steps and any increase in overall yield is difficult to obtain

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Conversion from D-Sorbitol to Vitamin C

[0032] Inoculate the G.oxydans DSM 4025 (FERM BP-3812) of an inoculating loop into the 5ml seed medium in the test tube, then cultivate it for 20 hours at 30°C with 240rpm on a reciprocating shaker, the G.oxydans DSM 4025 already contains 5.0% D-mannitol, 0.25% MgSO 4 ·7H 2 O, 1.75% corn steep liquor, 5.0% baker's yeast, 0.5% urea, 0.5% CaCO 3 and 2.0% agar medium were cultivated for 4 days at 27°C, and the seed medium contained 8.0% D-sorbitol, 5.0% baker's yeast, 0.05% glycerol, 0.25% MgSO 4 ·7H 2 O, 1.75% corn steep liquor, 0.5% urea, 1.5% CaCO 3 and a drop of defoamer.

[0033] 3 ml of seed culture was transferred to a 500 ml Erlenmeyer flask containing 50 ml of production medium containing 8.0% D-sorbitol, 5.0% baker's yeast, 0.05% glycerol, 0.25% MgSO 4 ·7H 2 O, 3.0% corn steep liquor, 1.5% CaCO 3 and 0.15% defoamer. Cultivation was carried out at 30° C. for 45 hours on a rotary shaker at 180 rpm. The...

Embodiment 2

[0034] Example 2: Conversion from L-sorbose to Vitamin C

[0035] Inoculate the G.oxydans DSM 4025 (FERM BP-3812) of an inoculating loop into the 5ml seed medium in the test tube, then cultivate it for 20 hours at 30°C with 240rpm on a reciprocating shaker, the G.oxydans DSM 4025 already contains 5.0% D-mannitol, 0.25% MgSO 4 ·7H 2 O, 1.75% corn steep liquor, 5.0% baker's yeast, 0.5% urea, 0.5% CaCO 3 and 2.0% agar medium were cultured for 4 days at 27°C, and the seed medium contained 8.0% L-sorbose, 5.0% baker's yeast, 0.05% glycerol, 0.25% MgSO 4 ·7H 2 O, 1.75% corn steep liquor, 0.5% urea, 1.5% CaCO 3 and a drop of defoamer.

[0036] 3 ml seed culture was transferred to a 500 ml Erlenmeyer flask containing 50 ml production medium containing 8.0% L-sorbose, 5.0% baker's yeast, 0.05% glycerol, 0.25% MgSO 4 ·7H 2 O, 3.0% corn steep liquor, 1.5% CaCO 3 and 0.15% defoamer. The cultivation was carried out at 30° C. for 20 hours on a rotary shaker at 180 rpm. As a resu...

Embodiment 3

[0037] Embodiment 3: From D-sorbitol, L-sorbose, L-sorbosone and L-oldo Lulose on the Production of Vitamin C

[0038] Containing 8.0% L-sorbose, 0.25% MgSO 4 ·7H 2 O, 1.75% corn steep liquor, 5.0% baker's yeast, 0.5% urea, 0.5% CaCO 3 G.oxydans DSM 4025 (FERM BP-3812) was cultured at 27°C for 4 days in an agar medium containing 2.0% agar. G. oxydans DSM 4025 (FERM BP-3812) grown on the above medium was transferred to 50 mM potassium phosphate buffer (pH 7.0), and washed twice with the same buffer. The optical density at 600 nm of the cell suspension was 21.9. Each ml of cell suspension contained 0.057 g of wet cells.

[0039] The reaction mixture (in test tube, 5 ml) contained the cell suspension and 8.0% D-sorbitol, 8.0% L-sorbose, 0.5% L-sorbosone or 1.0% L-gulose , the reaction mixture was in 50 mM potassium phosphate buffer (pH 7.0). Reactions were initiated by inoculating the cell suspension and performed at 30°C at 180 rpm on a reciprocating shaker. Vitamin C...

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PUM

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Abstract

The present invention provides a process for the production of vitamin C from different substrates like D-sorbitol, L-sorbose, L-sorbosone or L-gulose using a microorganism selected from the group consisting of Gluconobacter oxydans DSM 4025 (FERM BP-3812), a microorganism belonging to the genus Gluconobacter and having identifying characteristics of G. oxydans DSM 4025 (FERM BP-3812) and mutants thereof.

Description

technical field [0001] The present invention relates to the microbial production of L-ascorbic acid (vitamin C). Background technique [0002] Vitamin C, which is a very important and essential nutritional factor for humans, can be commercially produced by the so-called "Reichstein method", which is a well-known method that is technically mature. However, this method involves many complicated steps and any increase in overall yield is difficult to obtain. Therefore, many proposals have been made in an attempt to reduce the number of steps and / or increase the overall yield. Contents of the invention [0003] The present invention provides a method for producing vitamin C from D-sorbitol, L-sorbose, L-sorbosone or L-gulose by adding D-sorbitol, L-sorbose , L-sorbosone or L-gulose on the aqueous nutrient medium culture microorganisms to carry out, the microorganisms are selected from Gluconobacter oxydans DSM 4025 (FERM BP-3812) bacterial strains, with Gluconobacter oxydans...

Claims

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Application Information

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IPC IPC(8): C12P17/04C12P7/60
CPCC12P17/04C12P7/60
Inventor 星野达雄杉泽辉秀
Owner DSM IP ASSETS BV
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