Cell cryopreservation composition and cryopreservation method

Abstract

This composition can be easily added when cryopreserving cells and improves cell viability. 1 volume % of the composition containing 0.01 wt % to 20 wt % of a sophorose lipid is added just before or up to 6 hours before cryopreservation and cells are stored. This composition can improve cryopreservation cell viability as it reduces freezing damage to cells. With this composition, cells can be stored without using DMSO and blood serum. This is a simple and inexpensive storage method as it does not require fine control of the freezing rate during cell cryopreservation.

Description

TECHNICAL FIELD[0001]The present invention relates to cryopreservation compositions that are used to cryopreserve cells, and cryopreservation methods using the same.BACKGROUND ART[0002]Cell cryopreservation is widely used as an essential technique to prevent cell degeneration due to passaging, prevent contamination with bacteria associated with passaging, transport cells, etc. However, it is known that, in the process of freezing cells, water in and out of the cells turns into ice crystals and the ice crystals damage the cells (Non-Patent Document 1). It I therefore desired to protect cells from damage during freezing and thawing and to cryopreserve cells while maintaining their properties.[0003]In common cryopreservation, cells are typically suspended in a culture solution containing bovine serum etc. with a cryoprotectant for protecting the cells from damage from ice crystals, and the cell suspension thus obtained is placed in a cryotube etc., cooled, and eventually cryopreserved ...

AI Technical Summary

Benefits of technology

This patent describes a way to protect cells from damage caused by freezing. By adding a certain substance called SL, cells can be protected even without using other substances like DMSO and serum. This results in higher levels of cell survival.

Problems solved by technology

However, it is known that, in the process of freezing cells, water in and out of the cells turns into ice crystals and the ice crystals damage the cells (Non-Patent Document 1).

However, a cryopreservation solution containing DMSO as a cryoprotectant does not have satisfactory preservation efficiency, and it cannot necessarily be said that this cryopreservation solution sufficiently inhibits ice crystal formation.

However, it cannot be said that these cryopreservation solutions sufficiently inhibit ice crystal formation.

Moreover, since the cryopreservation solution contains as high as 30% of fructan, this is economically disadvantageous, and it is also difficult to remove fructan after preservation.

Cell preservation using carboxylated polylysine focuses only on stem cells and is therefore not so versatile.

The above cryopreservation solutions are thus not always satisfactory with respect to cell preservation.

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