Single cell capture with capture chips

Abstract

The present invention provides methods, systems, assemblies, and articles for capturing single cells with a capture chip. In certain embodiments, the capture chip comprises a substrate comprising a plurality of cell-sized dimples or wells that each allow a single cell to be captured from a cell suspension. In some embodiments, the dimples or wells of the capture chip align with the holes or wells of a multi-well through-hole chip, and / or a multi-well chip, such that the cell, or the contents of the single cell, may be transferred to a corresponding well of the multi-well chip. In particular embodiments, the bottom of each dimple or well of the capture chip has a positive electrical charge sufficient to attract cells from a cell suspension flowing over the dimples or wells.

Description

[0001]The present application is a Continuation-in-part of U.S. application Ser. No. 14 / 735,514 filed Jun. 10, 2015, now allowed, which claims priority to U.S. Provisional application 62 / 011,267, filed Jun. 12, 2014 and U.S. Provisional application 62 / 086,044, filed Dec. 1, 2014, all of which are herein incorporated by reference in their entireties.FIELD OF THE INVENTION[0002]The present invention provides methods, systems, assemblies, and articles for capturing single cells with a capture chip. In certain embodiments, the capture chip comprises a substrate comprising a plurality of cell-sized dimples or wells that each allow a single cell to be captured from a cell suspension. In some embodiments, the dimples or wells of the capture chip align with the holes or wells of a multi-well through-hole chip, and / or a multi-well chip, such that the cell, or the contents of the single cell, may be transferred to a corresponding well of the multi-well chip. In particular embodiments, the bot...

AI Technical Summary

Benefits of technology

Enables the efficient isolation and analysis of single cells, overcoming the limitations of traditional methods by allowing for the capture and processing of individual cells, which can then be lysed and have their nucleic acid sequenced, thereby revealing the clonal architecture of complex diseases.

Problems solved by technology

Geneticists are striving to characterize complex diseases like cancer, autoimmune and neurological disorders, but finding the underlying mechanisms driving these diseases has been elusive.

Sequencing bulk cell populations can mask the underlying heterogeneity of these unique rare cell types, making it difficult to distinguish them from normal germline mutations.

While single-cell sequencing can help uncover mechanisms of complex disease, traditional approaches are expensive, labor intensive, and require large sample input.

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