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Novel cheese and method for producing the same

a technology of cheese and cheese, applied in the field of new cheese, can solve the problems of social problems, side effects, headaches, etc., and achieve the effect of prevention and treatmen

Inactive Publication Date: 2015-08-20
SNOW BRAND MILK PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention is a cheese that contains specific amounts of angiogenin and cystatin, as well as a specific mass ratio between them. This cheese has a bone-strengthening effect and can be used to prevent and treat various bone diseases such as osteoporosis, fracture, rheumatism, and arthritis. The cheese can be made from milk or produced through genetic engineering. The patent also mentions that purified reagents made from angiogenin or cystatin can be used in the cheese. The technical effect of this patent is the development of a cheese that can be used for its bone-strengthening properties.

Problems solved by technology

In recent years, various bone diseases, such as osteoporosis, fracture, and backache have increased on a global basis along with aging of society and the like, and have become a serious social problem.
However, these drugs may bring side effects such as buzzing in the ear, a headache, or loss of appetite.
Moreover, the above substances are in a situation that they cannot be added to a food or drink at present from the viewpoint of safety, cost, and the like.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

reference example 1

Preparation (1) of Angiogenin Fraction

[0031]A column filled with 30 kg of cation-exchange resin (Sulfonated Chitopearl; manufactured by Fuji Spinning Co., Ltd.) was thoroughly washed with deionized water, and 1000 liters of unpasteurized skim milk (pH 6.7) was then applied to the column. After thoroughly washing the column with deionized water, the absorbed protein was eluted with a linear gradient of 0.1 to 2.0 M sodium chloride. The elution fraction containing angiogenin was fractionated using an S-Sepharose cation-exchange chromatography (manufactured by Amersham Bioscientific), and the resulted angiogenin-containing fraction was heat-treated at 90° C. for 10 minutes, and centrifuged to remove a precipitate. The angiogenin-containing fraction was further subjected to gel filtration chromatography (column: Superose 12). The eluate obtained was desalted using a reverse osmosis membrane, and the desalted eluate was freeze-dried to obtain 16.5 g of an angiogenin fraction having an an...

reference example 2

Preparation (2) of Angiogenin Fraction

[0032]A column filled with 10 kg of Heparin Sepharose (manufactured by GE Healthcare) was thoroughly washed with deionized water, and 500 liters of unpasteurized skim milk (pH 6.7) was then applied to the column. After thoroughly washing the column with a 0.5 M sodium chloride solution, the absorbed protein was eluted with a 1.5 M sodium chloride solution. The eluate was desalted using a reverse osmosis membrane, and the desalted eluate was freeze-dried to obtain 18 g of an angiogenin fraction having an angiogenin purity of 5%. The above successive operations were repeated 50 times.

reference example 3

Preparation of Cystatin Fraction

[0033]100,000 liters of a 5% whey protein solution was heat-treated at 90° C. for 10 minutes, and a precipitate was removed by centrifugation. A column was filled with a carrier prepared by binding carboxymethylated papain to Tresyl-Toyopearl (manufactured by Tosoh Corporation). After equilibration with a 0.5 M sodium chloride solution, the above whey protein solution was applied to the column. The column was then sequentially washed with a 0.5 M sodium chloride solution and a 0.5 M sodium chloride solution containing Tween 20 (0.1%). After that, a cystatin-containing fraction was eluted with a 20 mM acetic acid-0.5 M sodium chloride solution. The eluted fraction was immediately neutralized with a 1 M sodium hydroxide solution. The eluate was then desalted using a reverse osmosis membrane, and the desalted eluate was freeze-dried to obtain 9.6 g of a cystatin fraction having a cystatin purity of 90%. The above successive operations were repeated 20 ti...

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Abstract

The invention relates to a cheese includes angiogenin and / or angiogenin hydrolysate in an amount of 6.5 mg / 100 g to 160 mg / 100 g, and cystatin and / or cystatin hydrolysate in the mass ratio to the angiogenin and / or angiogenin hydrolysate of 0.02 to 1.6.

Description

TECHNICAL FIELD[0001]This invention relates to a novel cheese and a method for producing the same. The cheese includes a specific milk component, and may be useful for prevention and treatment of various bone diseases such as osteoporosis, fracture, rheumatism, and arthritis.BACKGROUND ART[0002]In recent years, various bone diseases, such as osteoporosis, fracture, and backache have increased on a global basis along with aging of society and the like, and have become a serious social problem. These diseases are caused by insufficient calcium intake, depression of calcium absorption ability, hormone imbalance after menopause, and the like. It is considered that increase the body bone mass as much as possible and increase the maximum bone mass and the bone strength (bone density+bone quality) by promoting osteoblastic bone formation from the early stage of life is effective in preventing various bone diseases, such as osteoporosis, fracture, and backache. Note that the term “bone qual...

Claims

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Application Information

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IPC IPC(8): A23C19/09A61K38/57A23L1/305A61K38/46A23L33/00
CPCA23C19/09A61K38/465A23V2002/00A23L1/3053C12Y301/27A61K38/57A23C19/053A23C19/082A23C19/0688A61K35/20A23V2200/306A23V2250/55A23V2200/00A61P19/08A61P19/10A23L33/00A23L33/18
Inventor OHMACHI, AIKOMATSUYAMA, HIROAKIMORITA, YOSHIKAZUISHIDA, YUKONARA, TAKAYUKIKATO, KENSERIZAWA, ATSUSHIUENO, HIROSHIURAZONO, HIROSHI
Owner SNOW BRAND MILK PROD CO LTD
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