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Pulmonary Surfactant Formulations

a technology of surfactant formulation and pulmonary vein, which is applied in the direction of drug composition, peptide, protein/protein ingredient, etc., can solve the problems of loss of functional surface tension activity, immunogenic reaction, and difficulty in purifying animal surfactants, so as to increase the aerosolization rate reduce the viscosity of drug delivery formulation, and increase the storage stability of drug delivery formulation

Inactive Publication Date: 2013-07-18
DISCOVERY LABORATORIES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention relates to a new pulmonary surfactant composition that has improved aerosol output rates and low viscosity compared to existing surfactants. The new surfactant contains dipalmitoyl phosphatidylcholine, phosphatidylglycerol, and a neutral lipid, and has no palmitic acid or 1-palmitoyl 2-oleoyl phosphatidylglycerol. The new surfactant also has a high degree of saturation and a low concentration of palmitoyl oleyl phosphatidylglycerol. The invention also provides a synthetic pulmonary surfactant that has improved aerosol output rates and low viscosity. The technical effects of the invention are improved performance and efficiency of the pulmonary surfactant in aerosol generation and reduced viscosity.

Problems solved by technology

Animal-derived surfactants are difficult to purify, limited by the scale to which they can be manufactured and have the potential to cause immunogenic reactions on repeat use, and thus are not indicated for use in other clinical indications.
However, this is at the expense of loss of functional surface tension activity as the content of the palmitic acid or cetyl alcohol is reduced as measured by in vitro techniques.
The current standard method of delivery does not instantaneously deliver the surfactant over the surface of the airways and alveoli.

Method used

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Examples

Experimental program
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Effect test

example 1

Development of Novel Lung Surfactant Formulation Containing DPPG and Cholesterol

[0257]Formulation of Lung Surfactant:

[0258]Lab-scale formulations were prepared at 60 mg / ml (TPL) with the cholesterol content variations described below (Table 3). Each formulation was prepared in n=1-5 (see lot numbers and names in Table 3) and individually characterized. The appropriate amounts (Table 3) of DPPC, POPG / DPPG, PA / cholesterol and KL4 by weight were sequentially added to a round bottom flask containing appropriate volumes of ethanol, which was heated to 45-50° C. Each of the components was added with 1-2 minutes application of bath sonication. Finally, after each of components was added, the flask was bath sonicated for 5 more minutes. The flask was attached to a rotary evaporator and the ethanol was evaporated at 55° C. The speed of the rotary evaporator was ˜50 rpm and a vacuum of <10 mbar was applied. When there was about 15 mls of ethanol remaining in the flask, the speed of the rotary...

example 2

Development of Novel Lung Surfactant Formulation Containing DPPG and Cholesterol-II

[0276]Formulation of Lung Surfactant.

[0277]Lab-scale formulations were prepared at 60 mg / ml (TPL) according to DOP-017, with the appropriate changes for cholesterol content variations described below (Table 4). Each formulation was prepared in formulation was prepared in triplicate (see lot numbers and names in Table 4) and individually characterized.

TABLE 4Formulation Lot Numbers, Names, andCholesterol Target ConcentrationsCholesterolFormulation LotConcentrationNameNumbermol (%)mg / mL2Ch1A0170-14-2Ch120.322Ch2A0170-14-2Ch220.322Ch3A0170-14-2Ch320.328Ch1A0170-15-8Ch181.268Ch2A0170-15-8Ch281.268Ch3A0170-15-8Ch381.2610Ch1A0170-14-10Ch1101.5810Ch2A0170-14-10Ch2101.5810Ch3A0170-14-10Ch3101.5812Ch1A0174-7-12Ch1121.8912Ch2A0174-7-12Ch2121.8912Ch3A0174-7-12Ch3121.8915Ch1A0174-7-15Ch1152.3615Ch2A0174-7-15Ch2152.3615Ch3A0174-7-15Ch3152.36

[0278]Analytical assays and procedures that were used to characterize the ...

example 3

In Vivo Activity of a Cholesterol-Containing Composition

[0288]The change in the volume of the respiratory system is measured by placing an animal, in this case a pre-term rabbit, in a plethysmograph, and measuring the change in volume of gas into and out of the plethysmograph with a flow meter, or pneumotachograph. The stiffness of the respiratory system is calculated by dividing the tidal volume change by the airway pressure change during a mechanical breath. The parameter for this stiffness is the compliance of the respiratory system (Crs).

[0289]In surfactant-deficient fetal-rabbits treated with surfactant, Crs is expected to increase during mechanical ventilation, and reach a plateau in approximately 10-30 minutes. This increase is due to the spreading of the surfactant and the establishment of a surface-active lining of the alveoli. In surfactant-deficient animals, there can be a small increase in the Crs with mechanical ventilation due to the release of minimal stores of surfac...

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Abstract

Synthetic pulmonary surfactant compositions comprising dipalmitoyl phosphatidylcholine, phosphatidylglycerol, and essentially neutral lipid, and having essentially no 1-palmitoyl 2-oleoyl phosphatidylglycerol and essentially no palmitic acid are provided. Methods for treating respiratory disease are also provided comprising administering a therapeutically effective amount of a synthetic pulmonary surfactant comprising dipalmitoyl phosphatidylcholine, phosphatidylglycerol, and essentially neutral lipid, and having essentially no 1-palmitoyl 2-oleoyl phosphatidylglycerol and essentially no palmitic acid.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority, under 35 U.S.C. §119(e), to application Ser. No. 60 / 638,665 filed Dec. 23, 2004, the disclosure of which is incorporated by reference in its entirety.FIELD[0002]The invention relates to a novel lung surfactant composition comprising a pulmonary surfactant and a neutral lipid that exhibits a reduction in surface tension, low viscosity characteristics and enhanced storage stability.BACKGROUND[0003]Pulmonary surfactants are complex lipid and protein compositions that can be extracted from animals, purified and used to treat neonatal respiratory distress syndrome. Animal-derived surfactants are difficult to purify, limited by the scale to which they can be manufactured and have the potential to cause immunogenic reactions on repeat use, and thus are not indicated for use in other clinical indications. To capitalize on the potential use of surfactants in a range of pulmonary disease states requires the availab...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K31/685A61K38/16
CPCA61K38/395C07K14/785A61K45/06A61K9/0082A61K31/575A61K31/683A61K38/16A61K31/685A61K2300/00A61P11/00
Inventor RAIRKAR, MAITHILIELIAZ, ROM E.NIVEN, RALPHJOHNSON, MARK E.
Owner DISCOVERY LABORATORIES INC
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