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Japanese blood fluke specific female fluke gene and its coded protein and application

A technology for specific protein and schistosome female, applied in the field of biological genes, can solve the problem of no public report on the specific protein of female adult Schistosoma japonicum, etc., and achieve the effect of preventing the spread

Inactive Publication Date: 2007-06-27
CHINESE NAT HUMAN GENOME CENT AT SHANGHAI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Before the present invention, there was no published report related to the female adult specific protein of Schistosoma japonicum of the present invention

Method used

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  • Japanese blood fluke specific female fluke gene and its coded protein and application

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Experimental program
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Effect test

Embodiment 1

[0031] Bioinformatic analysis of proteins specific to female adult worms of Schistosoma japonicum.

[0032] 1. Screening of specific proteins of Schistosoma japonicum female adults: using expressed sequence tag (EST) data to analyze specifically expressed proteins. The genes specifically expressed by females relative to males: 1) The significance of the difference must satisfy p<0.05, and this significance analysis is calculated using a special software tool on the Internet; 2) The number of ESTs for each gene must be greater than or equal to 10 ; 3) The number of ESTs of females must be 3 times or more than that of males. After the screening of these three criteria, the specific expression genes of female adults were obtained.

[0033] 2. Homology and similarity analysis: BLASTP comparison of the specific protein sequence of Schistosoma japonicum female adult to the non-redundant database (Non-redundant, NR) of GenBank (http: / / www.ncbi.nlm.nih.gov / ) Right; Schistosoma japon...

Embodiment 2

[0035] RT-PCR experiments verified the expression of specific proteins in Schistosoma japonicum female adults.

[0036] 1. Sample Preparation

[0037] Adult females and males of Schistosoma japonicum were prepared for RNA extraction.

[0038] 2. Total RNA extraction kit

[0039] The RNA extraction reagent uses TRIzol reagent (GIBCO / BRL), which is produced based on the one-step extraction method with acidic phenol. The utensils and water used for RNA extraction must be RNase-free to ensure an RNase-free environment in the experiment.

[0040] 3. RNA extraction steps

[0041] Dry-bake the pestle, homogenizer and other utensils at 200°C for 4 hours to remove RNase and cool down; add liquid nitrogen to pre-cool, take the tissue out of the liquid nitrogen quickly, and grind it into powder; use a spatula to put the tissue into the pre- Add TRIzol reagent to the homogenizer and homogenize for several minutes; transfer the homogenized liquid into an RNase-free centrifuge tube, add...

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Abstract

The present invention discloses Schistosoma japonicum specific female fluke protein and its gene sequence and specifically combined antibody as well as Schistosoma japonicum preventing and treating vaccine prepared with the specific female fluke protein as immunogen and the application of the specific female fluke protein in preparing medicine for preventing and treating Schistosoma japonicum. Applying the specific female fluke protein can inhibit the ovipositiom of female fluke to treat schistosomiasis and prevent Schistosoma japonicum diffusion.

Description

technical field [0001] The invention relates to the field of biological genes, in particular to the gene nucleic acid sequence of the specific protein of Schistosoma japonicum female adult and the amino acid sequence of the specific protein; in addition, the invention also relates to the application of the specific protein of the female adult Schistosoma japonicum. Background technique [0002] Schistosomiasis is caused by the infection of schistosomiasis. There are about 150 million infected people in 76 countries in the world. There are 3 kinds of schistosomes that cause disease, namely Schistosoma japonicum, Schistosoma mansoni (S.mansoni) and Schistosoma haematobium (S.haematobium). Schistosoma japonicum is prevalent in our country. The prevalence of schistosomiasis has caused huge losses to people's health and economic development. Therefore, the prevention and treatment of schistosomiasis and the development of drugs for the treatment of schistosomiasis and anti-schis...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12A61K38/17A61P43/00
CPCY02A50/30
Inventor 韩泽广刘锋王志勤胡薇冯正
Owner CHINESE NAT HUMAN GENOME CENT AT SHANGHAI
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