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Plant virus controlling sulfate polyose or oligose preparation and its preparing process

An anti-plant virus, anti-viral agent technology, applied in the directions of botanical equipment and methods, plant growth regulators, disinfectants, etc.

Inactive Publication Date: 2007-06-27
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the chemical synthesis of sulfated polysaccharides or oligosaccharides for resistance to plant virus diseases

Method used

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  • Plant virus controlling sulfate polyose or oligose preparation and its preparing process
  • Plant virus controlling sulfate polyose or oligose preparation and its preparing process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Chitin (1) (1 gram) was dissolved in pyridine (50 milliliters) at a temperature of 85 degrees, then added sulfur trioxide-pyridine complex (2.4 grams), reacted for 90 minutes at a temperature of 85 degrees, and cooled , the reaction mixture was neutralized to pH=7.5-8.0 with saturated barium hydroxide solution. The barium sulfate precipitate was removed, the mother liquor was treated with a sodium-type cation exchange resin column, and then the crude product was precipitated with acetone. After washing several times with acetone and freeze-drying, the sulfated chitin conjugate (2) (3.4 g) was obtained as a white powder.

[0015]

[0016] The efficacy test of the sulfated chitin conjugate (2) in the prevention and treatment of tobacco mosaic virus disease was carried out. The test object is tobacco mosaic virus (TMV for short); the test crop is tobacco, and the variety is "Common Tobacco Havana No. 38". The treatment concentrations of the test agent sulfated chitin ...

Embodiment 2

[0025] Chitin is chemically degraded to obtain acetylaminodextran oligosaccharide (3) (1 g) with a degree of polymerization of 2-30, dissolved in pyridine (50 ml) at a temperature of 85 degrees, and then added sulfur trioxide-pyridine complex (2.8 g) was reacted at 85°C for 90 minutes. After cooling, the reaction mixture was neutralized to pH=7.5-8.0 with saturated barium hydroxide solution. The barium sulfate precipitate was removed, the mother liquor was treated with a sodium-type cation exchange resin column, and then the crude product was precipitated with acetone. After washing several times with acetone and freeze-drying, the sulfated oligosaccharide conjugate (4) (1.6 g) was obtained as a white powder.

[0026] The efficacy test of the sulfated oligosaccharide conjugate (4) in preventing and treating tobacco mosaic virus disease was carried out. The test object is tobacco mosaic virus (TMV); the test crop is tobacco, and the variety is "common tobacco Havana No. 38". ...

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Abstract

The present invention is antiviral preparation of sulfate polyose or oligose conjugate with plant virus controlling function for protecting plant and its preparation process. The antiviral preparation is used for preventing and controlling viral diseases of different kinds of grain crop, economic crop, vegetable, fruit tree, flower, etc. It is prepared through chemically sulfatizing active polyose, including chitin polyose, algal polyose, konjack, etc. or active oligose as the natural polyose degrading product in the polymerization degree of 2-30.

Description

technical field [0001] An antiviral agent which can be used as a plant protection sulfated polysaccharide or oligosaccharide conjugate with anti-plant virus performance and its preparation method. Background technique [0002] Plant virus disease is a kind of important disease with various types, widespread occurrence and serious damage in agricultural production. It is called "plant cancer" because of its great harm and difficulty in prevention and control. After crops are infected by viruses, the economic losses cannot be accurately estimated; but there is no doubt that plant virus diseases have caused huge economic losses worldwide. [0003] Scientists have synthesized a large number of drugs and tested their antiviral activity. However, few cost-effective antiviral agents are practically used in agriculture. [0004] Recently, scientists have tested the activity of some anti-animal virus agents against plant viruses, and the results show that they also have anti-plant ...

Claims

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Application Information

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IPC IPC(8): A01N41/04A01P1/00
Inventor 宁君梅向东
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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