Producing method of functional quantum point used for specific recognizating tumor cell

A technology of tumor cells and quantum dots, applied in biological testing, material inspection products, etc., can solve problems such as non-specific staining, and achieve the effects of safe raw materials, safe and simple operation, and convenient preparation

Inactive Publication Date: 2007-06-06
NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Quantum dots used for biological research must have specific recognition functions for different types of cells. The existing quantum dots cannot meet this requirement in terms of surface functional modification, and there are non-specific staining problems in the dyeing process.

Method used

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  • Producing method of functional quantum point used for specific recognizating tumor cell
  • Producing method of functional quantum point used for specific recognizating tumor cell
  • Producing method of functional quantum point used for specific recognizating tumor cell

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Experimental program
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Effect test

Embodiment 1

[0026] 1. Preparation of quantum dots

[0027] (1) Prepare the solution of AOT and n-heptane

[0028] Prepare 30 mL of 0.1 M AOT solution in n-heptane.

[0029] (2) Configuration of reverse micellar system

[0030] Add CdCl to the above solution 2 aqueous solution, so that the concentration of the aqueous phase in the system is 0.1M. Add CdCl 2 After the aqueous solution was dispersed by ultrasonic waves for 30 minutes, until the system was clear and transparent, a reverse-phase micromicelle system was obtained.

[0031] (3) Reaction to generate CdS quantum dots

[0032] At room temperature, nitrogen gas was passed into the above-mentioned reversed-phase micromicelle system for 5 minutes to remove the oxygen in the system, and then under the condition of magnetic stirring, sufficient H was introduced into the above-mentioned micromicelle system. 2 S gas. When the color of the system no longer changes, it means that the reaction is complete and the quantum dots have been...

Embodiment 2

[0043] The greatest creativity of the method of the present invention lies in the step of functional modification of the surface of quantum dots (that is, linking of quantum dots and folic acid). This step is:

[0044] After the surface of the prepared quantum dots was treated by a small molecular compound containing mercapto groups, wash with acetone 3-5 times, wash, centrifuge, and dry alternately; -30 μL 0.05M NHS DMSO solution and the same volume of 0.05M EDC DMSO solution, stirred for 30-60 minutes, added 20-30 μL 0.05M folic acid DMSO solution, stirred, and reacted for 12-24 hours. Finally, the reactant was dialyzed with deionized water for 5-8 hours to remove unreacted small molecules.

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Abstract

A preparing method of the functional quanta point to identify the tumor cell specially belongs to the nanometer material and biological field. The method includes the below process: (1) preparing the CdS quanta point; (2) modifying the surface by small molecular compound with the mercapto; (3) the functional modification to the quanta point surface: add the DMSO solution with the NHS and the EDC to form a high activity midbody which can react with the amido in the folic acid. The mercapto can improve the water solution. So the fluorescent quanta point can identify the tumor cell expressing the high level folic acid acceptor, so it can be used as the fluorescent probe in cell biological study.

Description

Technical field [0001] A method for preparing functionalized quantum dots for specific recognition of tumor cells belongs to the field of nanometer materials and biotechnology of the present invention. Background technique [0002] Tumor is the number one disease that harms all human beings. Early diagnosis, early treatment and targeted therapy of tumors are the content that people have been paying attention to and researching. Traditional fluorescent dyes used in cell biology research have many limitations that cannot be overcome, such as strict requirements on excitation wavelength, low fluorescence efficiency, easy overlapping of emission wavelength and excitation wavelength, poor photostability, etc., which bring great challenges to biological research. A lot of inconvenience. [0003] With the development of technology, the research and development focus of nanotechnology has gradually shifted from the synthesis of a single nanomaterial to the function, application, a...

Claims

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Application Information

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IPC IPC(8): G01N33/53
Inventor 张培根余德才贺志鹏张海黔
Owner NANJING UNIV OF AERONAUTICS & ASTRONAUTICS
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