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Tissue cultured seedling water medium domestication technology

A technology of tissue culture seedlings and water medium, which is applied in horticultural methods, botany equipment and methods, plant phenotype improvement, etc. It can solve the problems of poor growth consistency of tissue culture seedlings and unsatisfactory survival rate of tissue culture seedlings, and achieve domestication The effect of small occupied area, simple and easy technical process operation, and short domestication cycle

Inactive Publication Date: 2010-11-03
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the "patch" effect of the environment in the above domestication process is more obvious, resulting in poor growth consistency of tissue cultured seedlings, and the survival rate of tissue cultured seedlings in the domestication process is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1), put the container containing the tissue culture seedlings into the acclimatization room, select scattered light as the light source in the acclimatization room, and control the light intensity at 3500 lux, open the container port with the tissue culture seedlings after 45 hours of treatment, and add an equal amount of 0.05% polysaccharide successively. The amount of betazolin solution and 0.10% streptomycin solution was controlled at a thickness of 3-4 cm covering the surface of the medium, the ambient temperature of the acclimatization room was controlled at 25°C during the day, 12°C at night, and the relative air humidity was controlled at 80%. sky;

[0018] 2) A sponge with a height of 1-2cm is placed at the bottom of the transparent container;

[0019] 3) Then take out the tissue culture seedlings and place them in a transparent container with a sponge. Put 12-15 tissue culture seedlings in each bottle, add tap water to a height of 3-5 cm at the bottom of the tr...

Embodiment 2

[0024] 1), put the container containing the tissue cultured seedlings into the acclimation room, select scattered light as the light source in the acclimation room, and control the light intensity at 3000 lux, open the container port containing the tissue cultured seedlings after 50 hours of treatment, and add an equal amount of 0.1% polysaccharide successively. The amount of betazolin solution and 0.05% penicillin solution is controlled to cover the thickness of the medium surface by 3-4cm, the ambient temperature of the acclimatization room is controlled at 20°C during the day, 15°C at night, and the relative air humidity is controlled at 72%. Culture for 6 days;

[0025] 2) Set rock wool with a height of 1-2cm at the bottom of the transparent container;

[0026] 3) Then take out the tissue culture seedlings and place them in a transparent container with a sponge. Put 12-15 tissue culture seedlings in each bottle, add distilled water to a height of 3-5 cm at the bottom of the...

Embodiment 3

[0031] 1), put the container containing the tissue culture seedling into the acclimatization room, select scattered light as the light source in the acclimatization room, and control the light intensity at 5000 lux, open the container port with the tissue culture seedling after 55 hours of treatment, and add an equal amount of 0.04% polysaccharide successively. The amount of betazolin solution and 0.12% streptomycin solution was controlled at a thickness of 3-4 cm covering the surface of the medium, the ambient temperature of the acclimation room was controlled at 18°C ​​during the day, 14°C at night, and the relative air humidity was controlled at 75%. sky;

[0032] 2) A sponge with a height of 1-2cm is placed at the bottom of the transparent container;

[0033] 3) Then take out the tissue culture seedlings and place them in a transparent container with a sponge. Put 12-15 tissue culture seedlings in each bottle, add tap water to a height of 3-5 cm at the bottom of the transp...

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Abstract

The invention discloses a taming technique of tissue cultivating sprout water-dielectric, which comprises the following steps: loading tissue cultivating sprout container in the taming chamber; adding fungal inhibiting agent and bacterium inhibiting agent; controlling light, temperature, air moisture to cultivate; placing tissue cultivating sprout in the transparent container with sponge or rock wool; adding taming base; controlling temperature and air moisture.

Description

technical field [0001] The invention relates to a water medium domestication technology of tissue cultured seedlings. Background technique [0002] Since tissue culture seedlings are grown under the conditions of sterility, nutrient supply, suitable light intensity and temperature, and near-saturated relative humidity, their physiological and ecological characteristics are very different from those of seedlings grown in natural conditions: tissue culture seedlings There is no waxy layer on the surface of the leaves, few or no epidermal appendages, the opening of stomata cannot be closed normally, the mesophyll palisade tissue is disordered, and the chlorophyll content is low; the root system has no root hairs, and the root system and the vascular system between the root system and the plant are poorly developed. Therefore, the survival rate of tissue-cultured seedlings directly transplanted is very low, and it is necessary to make corresponding changes in physiology, shape, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01H3/00
Inventor 吕国华贾晓鹰李文凯权俊萍
Owner SHIHEZI UNIVERSITY
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