Tissue cultured seedling water medium domestication technology
A technology of tissue culture seedlings and water medium, which is applied in the fields of plant phenotype improvement, horticulture, and botanical equipment and methods. The effect of small area, simple and easy technical process operation, and short domestication period
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Embodiment 1
[0017] 1), put the container containing the tissue culture seedlings into the acclimatization room, select scattered light as the light source in the acclimatization room, and control the light intensity at 3500 lux, open the container port with the tissue culture seedlings after 45 hours of treatment, and add an equal amount of 0.05% polysaccharide successively. The amount of betazolin solution and 0.10% streptomycin solution was controlled at a thickness of 3-4 cm covering the surface of the medium, the ambient temperature of the acclimatization room was controlled at 25°C during the day, 12°C at night, and the relative air humidity was controlled at 80%. sky;
[0018] 2) A sponge with a height of 1-2cm is placed at the bottom of the transparent container;
[0019] 3) Then take out the tissue culture seedlings and place them in a transparent container with a sponge. Put 12-15 tissue culture seedlings in each bottle, add tap water to a height of 3-5 cm at the bottom of the tr...
Embodiment 2
[0024] 1), put the container containing the tissue cultured seedlings into the acclimation room, select scattered light as the light source in the acclimation room, and control the light intensity at 3000 lux, open the container port containing the tissue cultured seedlings after 50 hours of treatment, and add an equal amount of 0.1% polysaccharide successively. The amount of betazolin solution and 0.05% penicillin solution is controlled to cover the thickness of the medium surface by 3-4cm, the ambient temperature of the acclimatization room is controlled at 20°C during the day, 15°C at night, and the relative air humidity is controlled at 72%. Culture for 6 days;
[0025] 2) Set rock wool with a height of 1-2cm at the bottom of the transparent container;
[0026] 3) Then take out the tissue culture seedlings and place them in a transparent container with a sponge. Put 12-15 tissue culture seedlings in each bottle, add distilled water to a height of 3-5 cm at the bottom of the...
Embodiment 3
[0031] 1), put the container containing the tissue culture seedlings into the acclimatization room, select scattered light as the light source in the acclimatization room, and control the light intensity at 5000 lux, open the container port with the tissue culture seedlings after 55 hours of treatment, and add an equal amount of 0.04% polysaccharide successively. The amount of betazolin solution and 0.12% streptomycin solution was controlled at a thickness of 3-4 cm covering the surface of the culture medium, the ambient temperature of the acclimatization room was controlled at 18°C during the day, 14°C at night, and the relative air humidity was controlled at 75%. sky;
[0032] 2) A sponge with a height of 1-2cm is placed at the bottom of the transparent container;
[0033] 3) Then take out the tissue culture seedlings and place them in a transparent container with a sponge. Put 12-15 tissue culture seedlings in each bottle, add tap water to a height of 3-5 cm at the bottom...
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