Test paper bar for testing colloidal gold of respiratory tract syncytial virus antigen

Abstract

This invention provides one test bar, which comprises the following steps: a, covering syncytium virus antibody of respiratory tract and IgG two band NC fiber film; b, comprising glue gold label respiratory syncytium virus single clone antibody glass fiber film; b, applying film analysis double antibody clamper principle to test nose secretion respiratory virus.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a respiratory syncytial virus (RSV) antigen detection test strip and an application thereof. Background technique [0002] In the 1970s, the diagnosis of RSV at home and abroad was mainly virus isolation, but because many cells were not sensitive to RSV, the operation was cumbersome and complicated, and it took a long time, so virus isolation was difficult to be an effective method for RSV infection; in rapid detection, polyclonal antiserum was used The IFA (indirect fluorescence technique) method is used to detect RSV antigen in the exfoliated cells of the nasopharynx of patients. This method has good sensitivity, but there is non-specific staining, which greatly affects the specificity of the method. Monoclonal antibodies came out in the 1980s, and Bell and Kim used monoclonal antibodies to detect RSV antigens by IFA method and achieved satisfactory results, whic...

AI Technical Summary

Problems solved by technology

[0002] In the 1970s, the diagnosis of RSV at home and abroad was mainly virus isolation, but because many cells were not sensitive to RSV, the operation was cumbersome and complicated, and it took a long time, so virus isolation was difficult to be an effective method for RSV infection; in rapid detection, polyclonal antiserum was used The IFA (indirect fluorescence technique) method is used to detect the respiratory syncytial virus antigen in the exfoliated cells of the patient's nasopharynx. This method has good sensitivity, but there is non-specific staining, which greatly affects the specificity of the method.

With the continuous development of immune technology, APAAP bridged enzyme labeling method and nucleic acid amplification method (PCR) have appeared successively, but there are problems such as requiring expensive instruments, complicated operation, long time, and professional personnel.

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