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Industrial production method for high-purity low molecular weight soybean obligopeptide powder

A soybean oligopeptide, high-purity technology, applied in protein food processing, vegetable protein processing, protein food ingredients and other directions, can solve the problems of difficult separation methods, difficult processing, realization, etc., to reduce hypertension, reduce blood lipids, enhance immune effect

Active Publication Date: 2007-03-28
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method adopts isoelectric point precipitation and separation, and needs to add a lot of hydrochloric acid, which will make subsequent processing difficult and affect the taste of the product. In addition, it is also a small test and cannot be ind

Method used

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  • Industrial production method for high-purity low molecular weight soybean obligopeptide powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Add 6000L deionized water and 500kg soybean protein isolate powder to a 10000L hydrolysis tank with a water interlayer, and add materials while adding water to prevent agglomeration from too fast mixing. Stir well, and adjust the pH to 7.0-9.0 with NaOH solution. Add 10kg of compound protease, and after enzymolysis at 40-60°C for 6 hours, heat the enzymolysis solution at 135°C for 15 seconds. The degree of hydrolysis of protein reaches 55%. The soybean protein enzymatic hydrolyzate is separated by a combined separation system, that is, centrifuged by a disc separator to obtain pulp residue and centrifugal supernatant. Collect the supernatant for later use. Use a ceramic membrane microfiltration device with a pore size of 0.1 μm-0.5 μm for filtration and separation to obtain the retentate and permeate, and collect the permeate for future use. Finally, filter the ultrafiltration permeate obtained in the previous step with a hollow fiber membrane ultrafiltration device ...

Embodiment 2

[0045] Take 800kg soybean protein isolate as raw material, add protease and carry out enzymolysis processing according to the ratio of enzyme used in Example 1, finally obtain 380kg soybean oligopeptide powder. The analysis results show that the soybean peptide content in the product: 81.3%; the free amino acid content: 2.4%.

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Abstract

The present invention relates to an industrial production method of soybean oligopeptide with high-purity and low molecular weight. Said invention adopts soybean protein isolate (original protein) as raw material and makes the raw material undergo the processes of enzymatic hydrolysis, separation, concentration, decoloring and spray-drying so as to obtain the invented soybean digopeptide powder which can be used as nutrient additive of health-care food.

Description

technical field [0001] The invention relates to a high-purity soybean oligopeptide industrial product, the soybean peptide content of which is more than 85%, and the molecular weight of 90% of the soybean peptide is below 1000Dalton. The invention also relates to limited enzyme-catalyzed hydrolysis of soybean protein isolate to produce soybean oligopeptide powder, which is used as raw material of health food and common food. The invention also relates to a method for industrial production of the soybean oligopeptide. Background technique [0002] Soy protein isolate is a high-quality vegetable protein with multiple functional properties, such as gelling, emulsifying, and water-holding properties. Soy protein isolate is widely used as a food ingredient. On the other hand, soybean protein isolate, as soybean original protein, has certain antigenicity, which is easy to cause people, especially children, to be allergic. When using soybean protein isolate to prepare acidic soy...

Claims

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Application Information

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IPC IPC(8): A23L1/305A23J3/16A23J3/34A23L33/18
Inventor 蔡木易易维学谷瑞增董哲
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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