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Method for preparing analogue of carnosine

A technology of analogues and carnosine, which is applied in the field of preparation of carnosine analogues, can solve the problems of increasing the protection of amino and carboxyl groups and removing amino and carboxyl groups respectively, which is unfavorable for industrial batch production and hinders popularization and application, and achieves the reduction of production Low cost, easy industrial production, strong specific effect

Inactive Publication Date: 2007-02-21
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the generation rate of carnosine is high, it increases the steps of protecting amino and carboxyl groups and removing amino and carboxyl groups respectively, and produces a large amount of organic wastewater containing protecting groups and condensation agent derivatives to pollute the environment
Another example is the patent No. ZL200510094045 "cerebroside carnosine freeze-dried powder and its preparation method" which discloses the direct extraction of small molecular polypeptides from rabbit meat. Since the carnosine content is only a few millimoles per kilogram of muscle, the cost is extremely high and the product purity is low. , can only be used to prepare condiments, which is not conducive to industrial mass production and hinders popularization and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The concrete steps of a kind of carnosine analog preparation method are as follows:

[0025] (1) Preparation of synthetic carnosine analogue solvent

[0026] In the tetrahydrofuran solution, add a phosphate buffer solution with a pH value of 8.5 and a concentration of 0.1mol / L. The volume ratio of the phosphate buffer solution: tetrahydrofuran solution is 1: 1000. After stirring evenly, a synthetic phosphate buffer solution is prepared. Carnosine analog tetrahydrofuran solvent.

[0027] (2) prepare the substrate solution of synthetic carnosine analog

[0028] According to the molar ratio of 4,5 dicarboxylic acid imidazole:L-alanine is 1:3 ratio, after 4,5 dicarboxylic acid imidazole and L-alanine are mixed and stirred evenly, then according to 4,5 two The total weight of carboxylic acid imidazole and L-alanine: the ratio of distilled water volume is 1:1, add distilled water and stir to dissolve, and just prepare the substrate solution of synthetic carnosine analogue. ...

Embodiment 2

[0040] The concrete steps of a kind of carnosine analog preparation method are as follows:

[0041] Preparation of Synthetic Carnosine Analog Solvents

[0042] (1) In the tetrahydrofuran solution, add a phosphoric acid buffer solution with a pH value of 8.8 and a concentration of 0.1mol / L, and the volume ratio of the phosphoric acid buffer solution: tetrahydrofuran solution is 1: 1200. After stirring evenly, a phosphoric acid buffer solution is prepared. Liquid Synthesis of Carnosine Analogs in THF Solvent.

[0043] (2) prepare the substrate solution of synthetic carnosine analog

[0044] According to the molar ratio of 4,5 dicarboxylic acid imidazole:L-alanine is 1:3.2, after mixing 4,5 dicarboxylic acid imidazole and L-alanine and stirring evenly, then according to 4,5 dicarboxylic acid imidazole The total weight of carboxylic acid imidazole and L-alanine: the ratio of volume of distilled water is 1:1.3, add distilled water and stir to dissolve, just prepare the substrate ...

Embodiment 3

[0056] The concrete steps of a kind of carnosine analog preparation method are as follows:

[0057] (1) Preparation of synthetic carnosine analogue solvent

[0058] In the tetrahydrofuran solution, add a phosphate buffer solution with a pH value of 9.0 and a concentration of 0.1mol / L. The volume ratio of the phosphate buffer solution: tetrahydrofuran solution is 1: 1250. After stirring evenly, a synthetic phosphate buffer solution is prepared. Carnosine analog tetrahydrofuran solvent.

[0059] (2) prepare the substrate solution of synthetic carnosine analog

[0060] According to the molar ratio of 4,5 dicarboxylic acid imidazole:L-alanine is 1:3.5, after mixing 4,5 dicarboxylic acid imidazole and L-alanine and stirring evenly, then according to 4,5 dicarboxylic acid imidazole The total weight of carboxylic acid imidazole and L-alanine: the ratio of volume of distilled water is 1:1.5, add distilled water and stir to dissolve, just prepare the substrate solution of synthetic c...

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Abstract

This invention discloses a method for preparing carnosine analog. The method uses enzyme as the catalyst, and does not need group protection or condensation agent during the reaction, thus having such advantages as simple process, mild reaction conditions, easy operation, complete utilization of resources, no pollution and low cost. The prepared canosine analog has high purity (93.5-95.5%), good quality and strong antioxidative effect, and can be used as antioxidative active substances in medicine, food, health product, cosmetic and feed.

Description

1. Technical field [0001] The invention belongs to the technical field of preparation of medicinal antioxidant active substances, in particular to a preparation method of carnosine analogues. 2. Background technology [0002] Carnosine is a dipeptide composed of the α-carboxyl group of β-alanine and the α-amino group of L-histidine through an amide bond. It has physiological functions such as anti-oxidation, free radical scavenging, and pH buffering. The biological activity of carnosine is mainly due to the -N on the imidazole ring 1 H-and-N 3 -As a result of the conjugate acid and its base action, therefore, the imidazole-containing carnosine analogues also have biological activity. It can be widely used in medicine, food, health products, cosmetics, feed and other fields. [0003] The existing methods for preparing carnosine include chemical synthesis and direct extraction from muscle. Such as the chemical synthesis method disclosed in "A Production Method of Carnosine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D233/64
Inventor 周小华陆瑶左瑞雅
Owner CHONGQING UNIV
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