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Relativity between AnnexinA3 and drug resistance of platinum type chemical curing medication for cancer

A chemotherapeutic drug and drug resistance technology, which is applied in the field of the correlation between Annexin A3 and platinum-based chemotherapeutic drug resistance in cancer, can solve the problem of no research report on the correlation of tumor chemotherapy resistance.

Inactive Publication Date: 2007-02-21
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no research report on the correlation between annexin A3 and tumor chemotherapy resistance

Method used

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  • Relativity between AnnexinA3 and drug resistance of platinum type chemical curing medication for cancer
  • Relativity between AnnexinA3 and drug resistance of platinum type chemical curing medication for cancer
  • Relativity between AnnexinA3 and drug resistance of platinum type chemical curing medication for cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0175] In the study of the present application, the inventors of the present application used proteomics technology for the first time to study the differences in protein expression profiles between drug-resistant and sensitive cells, and then searched for possible drug resistance markers.

[0176] Firstly, the ovarian cancer cell line SKOV3 resistant to cisplatin induced by the high-dose pulse method and the low-dose intermittent method was established. Through the detection of the biological characteristics of the two cell lines, it was found that different induction methods of the same drug have different effects on resistance to cisplatin. The expression of drug genes has different effects, and the small-dose intermittent method is more likely to produce drug resistance. And it has established a good cell model for studying the mechanism of platinum resistance.

[0177] Secondly, two ovarian cancer sensitive cell lines, SKOV3 and A2780, and four drug-resistant cell lines, ...

Embodiment 1

[0180] Example 1 Establishment and identification of epithelial ovarian cancer drug-resistant cell lines

[0181] (1) Experimental method

[0182] 1) Cell lines and cell culture

[0183] The human serous ovarian cancer SKOV3 cell line was purchased from the Basic Medical Cell Center of the Chinese Academy of Medical Sciences. The cells were grown adherently in DMEM (HG) (Gibco, USA) medium containing 10% fetal bovine serum, placed at 37°C, 5% CO 2 Cultured in a saturated humidity incubator, digested and passaged with 0.25% trypsin.

[0184] 2) Establishment of drug-resistant cell lines

[0185] 1. The high-dose shock induction method is named as SKOV3 / CDDP-P cells. After adding 100 μmol / L CDDP (cisplatin, FH Keding Co., Ltd.) to the medium for 2 hours, discard the drug-containing medium, add drug-free medium, and place at 37°C, 5% CO 2 Continue culturing in a saturated humidity incubator until the cells grow to the logarithmic growth phase (density reaches 70%-80%), and r...

Embodiment 2

[0249] Example 2 Screening and Validation of Platinum Resistance-Related Proteins in Epithelial Ovarian Cancer

[0250] (1) Experimental method

[0251] 1) Cells and cell culture: human serous ovarian cancer cell line SKOV3 was purchased from the Basic Medical Cell Center of the Chinese Academy of Medical Sciences. The SKOV3 / CDDP line was induced into drug-resistant cell lines by high-dose cisplatin pulse method in our laboratory (see the first part for the specific induction method. Since the high-dose pulse induction method is similar to the clinical chemotherapy method, SKOV3 / CDDP was used in subsequent studies. -P is the research object and named as SKOV3 / CDDP). Human epithelial ovarian cancer cell lines A2780, A2780 / CDDP, A2780 / CBP and SKOV3 / CBP were provided by Professor Li Li (Department of Gynecologic Oncology, Cancer Hospital Affiliated to Guangxi Medical University). All cells were grown adherently in DMEM (HG) medium containing 10% fetal bovine serum, placed at 37...

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Abstract

An association between annexin A3 and the resistance of cancer to the chemicotherapeutic Pt medicines, a method for increasing or decreasing said resistance by changing the annexin A3 expression in cells, a method for testing or diagnosing said resistance, the medicines used for said methods and their components, usage and screening method, and the therapeutic method for the cancers, especially ovarian cancer, are disclosed.

Description

technical field [0001] The present invention generally relates to the field of chemotherapeutic drug resistance, and more specifically the present invention relates to the correlation between annexin A3 and the resistance of platinum-based chemotherapeutic drugs in cancer, and relates to increasing / decreasing platinum in cancer by changing the expression of annexin A3 in cells A method for drug resistance to chemotherapeutic drugs, and a medicament that can be used in the method to change the expression of annexin A3 in cells, a pharmaceutical composition comprising the medicament, and the use of the medicament for preparing a drug that changes drug resistance of cancer, and A screening method for the medicament. In addition, the present invention also relates to detection or diagnosis methods, medicaments, pharmaceutical compositions and kits for detection and / or prognosis of cancer resistance to platinum-based chemotherapy drugs. The invention also relates to methods for tr...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K31/282A61K31/555C12N15/09C12N15/85C12N15/12C12N9/22C07K16/18A61P35/00
Inventor 潘凌亚闫雪冬
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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