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Method for preparing high purity alpha corn protein

A high-purity technology of corn protein, applied in the field of development and utilization of plant protein, to achieve a wide range of utility and functional effects

Inactive Publication Date: 2007-01-24
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above reports show that the current common methods generally have contradictions between decolorization, yield and product performance, and decolorization is a major factor restricting the development of corn gluten meal deep processing technology

Method used

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  • Method for preparing high purity alpha corn protein

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 100g corn gluten powder (water content 5%) was granulated, then added 5 times the volume of absolute ethanol, defatted twice; defatted corn gluten granules were added 6 times the volume of 80% ethanol, leached in countercurrent at room temperature for 24 hours, and solid-liquid separation; Add 2 times the volume of 0.25% NaCl aqueous solution to the filtrate, and wash the obtained precipitate with water three times repeatedly to remove the NaCl therein. Carry out vacuum drying then and make yellow zein 28g.

Embodiment 2

[0023] 100g corn gluten powder (water content 5%) was granulated, then added 5 times the volume of absolute ethanol, defatted twice; defatted corn gluten granules were added 6 times the volume of 80% ethanol, leached in countercurrent at room temperature for 24 hours, and solid-liquid separation; Adjust the pH of the filtrate to 5.5, add 10% activated carbon powder, and stir at 30°C for 5 hours; use high-speed centrifugation for solid-liquid separation of the decolorization solution; concentrate the filtrate to 1 / 2 volume under reduced pressure, recover ethanol, and then vacuum Dry to obtain 25g of white corn protein.

Embodiment 3

[0025] 100g of corn gluten powder (water content 5%) was granulated, then added 7 times the volume of absolute ethanol, defatted twice; defatted corn gluten granules were added with 10 times the volume of 90% ethanol, leached in countercurrent at room temperature for 12 hours, and separated into solid and liquid; Adjust the pH of the filtrate to 6.5, add 6% activated carbon powder, and stir at 60°C for 2 hours; the decolorization solution is separated from solid and liquid by vacuum filtration; 3 times the volume of 1% NaCl aqueous solution is added to the filtrate, and the obtained precipitate is washed repeatedly with water for 3 Once, the NaCl therein was removed completely; then vacuum drying was carried out to obtain 27g of white α-zein.

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Abstract

The present invention provides a kind of high purity alpha-corn protein preparing process. The preparation process includes the following steps: extracting alpha-corn protein from corn protein powder with 90 % concentration ethanol solution in a countercurrent extraction process; the subsequent regulating the temperature and pH value of ethanol solution of alpha-corn protein, and adding active carbon through stirring to decolorize; and the final closed-path spray drying under the protection of nitrogen to obtain high quality alpha-corn protein. The decolorized and deodorized alpha-corn protein may be used safely in food and medicine industry.

Description

technical field [0001] The invention relates to the field of development and utilization of plant protein, in particular to a method for preparing high-purity, high-quality and low-cost α-corn gluten by using corn gluten powder as a raw material. It can be widely used in food, medicine and packaging industries. Background technique [0002] A by-product, corn gluten meal CGM (CornGluten Meal), is obtained in the process of wet corn starch production, with a protein content of about 60%. At present, corn gluten meal is mainly sold as feed, with low added value. Corn gluten meal contains about 30-35% α-zein. This protein has been widely used in food, papermaking, printing, textile, medicine and hygiene in recent years because of its good water resistance, oxygen resistance and easy film formation, especially for food packaging. It is a kind of protein with broad application prospects. coating material. [0003] Due to the presence of lutein, carotenoids and other pigments ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/12
Inventor 董英徐春芳徐斌查青张红印
Owner JIANGSU UNIV
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