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Tooth steam cell and its production

A technology of stem cells and teeth, applied in the field of stem cells, can solve the problem of undiscovered dental epithelial stem cells, and achieve the effect of convenient and feasible material acquisition and excellent biological characteristics

Inactive Publication Date: 2007-01-17
叶庆良
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Evidence for dental epithelial stem cells has long been absent from the study of differentiated ameloblasts

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1. Source of Stem Cells

[0018] A 13-year-old male, the second permanent molars have erupted, but the third permanent molars have not yet erupted. The X-ray film confirmed that the third permanent molar tooth germ has been formed. The preventive third permanent molar tooth germ was removed through surgery, and a total of 4 The tooth germs were preserved at 4°C in physiological saline containing 100 IU / ml gentamycin.

Embodiment 2

[0019] Example 2. Collection of Stem Cells

[0020] Collection of dental papilla specimens: samples were collected under sterile conditions, and the tooth germ tissue obtained in Example 1 was placed in D-Hanks solution. Use gentamicin containing 100IU / ml for flushing to wash away residual blood stains in the tissues and disinfect them. Under sterile conditions, the tissues of the dental papilla were excised and placed in DMEM / F12 medium solution.

Embodiment 3

[0021] Example 3. Primary culture of stem cells

[0022] Primary culture of cells was performed within 12 hours after sampling. The obtained tissue pieces were cut into pieces, blown repeatedly with a straw, and mechanically separated. Centrifuge at 1500 rpm for 10 minutes. Discard the supernatant, add DMEM / F12 medium containing endothelial cell growth factor (ECGF) 10ng / ml and 10% fetal calf serum, 5% CO 2 , Culture at 37°C. at 35mm 2 Plastic Petri dish or 25mm 2 Culture flask culture, adjust the cell concentration to 1~2×10 6 / ml.

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PUM

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Abstract

A tooth dry cell and its production are disclosed. The tooth dry cell is selected from dentary germ of the third accessional teeth or the third erupted accessional teeth. It can be used to repair damaged teeth and reproduce new teeth. It is simple, convenient and has excellent biological activity.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to an adult stem cell, especially a dental stem cell and a preparation method thereof. Background technique [0002] Stem cell (stemcell) research is one of the most eye-catching hot spots in the field of medicine and biology since the 1990s. It is used as an in vitro model to study the development mechanism of individuals, and it is used in cell replacement therapy and gene therapy for clinical tissue defect diseases. It has great application value. [0003] Stem cells are cells from embryos, fetuses or adults with the capacity for permanent or lifelong self-renewal, which give rise to specialized cell types that form human tissues and organs. At present, the research on stem cells can generally be classified according to the following two methods. One is to divide them into three categories according to their differentiation ability: 1) totipotent stem cells; 2) pluripotent stem cells; ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N5/074
Inventor 叶庆良
Owner 叶庆良
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