Method of making cell growth surface

Abstract

The present invention discloses a method of making cell growth surface, which comprises a three-dimensional porous growth surface made from polysaccharide material, especially the alginic acid, to enhance cell growth surface, promote cell adherence, immobilization and propagation, maintain surface structure integrity, enable programmable degradation, and thus increase cellular production. The present invention teaches several methods: a method to enhance the integrity of the growth surface by protecting the growth surface in a rigid solid support; a method of use for enhancing the performance of the surface; and a method of modifying a growth surface for eukaryotic and / or prokaryotic cells comprising the steps of increasing surface area by creating porous and 3-D structure, treating a surface to encourage cell attachment, promoting cell growth and proliferation, and disposing the growth surface in any conventional cell cultivating device. The growth surface is able to program degradation and release the cell / tissue mass after the culture is completed.

Description

technical field [0001] The invention provides a growth surface, a cell culture structure and a manufacturing method, especially a growth surface capable of harvesting a large number of cells, and a cell culture manufacturing method. Background technique [0002] The revolutionary progress of biotechnology and genetic engineering has created a high market demand for cell products, such as protein drugs, cytokines, interferons, monoclonal antibodies, hormones, growth factors, insulin ( Insulin), viral products, vaccines, nucleic acids, enzymes, and transplanted cells or tissues. The market demand for this product thus drives the need for economical and efficient mass production methods. [0003] Eukaryotic cells (Eukaryotic cells), such as mammalian cells, have become more and more common in the production of high-quality protein products. The use of cultured mammalian cells to produce vaccines, genetically engineered proteins, drugs and other cell products has continued For...

AI Technical Summary

Problems solved by technology

[0023] In order to solve the above-mentioned shortcomings of the carrier formed by alginic acid (or alginate), including: cell density and availability are limited during culture, most of the adherent cells cannot adhere or grow on the surface of alginate, and many The structural strength of the porous alginate carrier is too low; the present invention adds additional calcium ions to the medium to surround the surface of the alginate hydrogel so that the adherent cells can adhere, expand and grow at a comparable growth rate and density, protecting the porous alginate The gel matrix is ​​in a network, and the integrity of the porous culture matrix can be maintained for a long period of time during cell culture

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