Soybean isoflavone extract, its preparation process and pharmaceutical compositions containing same

A technology of soybean isoflavones and extracts, applied in sugar derivatives, pharmaceutical formulations, plant raw materials, etc., can solve the problems of repeated resin treatment and regeneration, organic solvent residue, cumbersome operation, etc.

Inactive Publication Date: 2006-12-13
北京云威科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] CN1422855A and CN1422856A disclose the method of ethanol extraction of soybean meal and adsorption by macroporous adsorption resin to purify soybean isoflavones. The disadvantages of these methods are: (1) resin residue; (2) desorption brings organic solvent residue; (3) The resin needs repeated treatment and regeneration
But the disadvantages are: (1) Silica gel column chromatography is not easy to be industrialized; (2) There are environmental problems in using a large amount of silica gel; (3) The product cost is high
The shortcoming of this method is: (1) resin residue; (2) organic solvent residue; (3) resin needs repeated treatment and regeneration; (4) silica gel column chromatography is difficult for industrialization; (5) there is environmental protection problem in a large amount of using silica gel; 6) The production cycle is long and the operation is cumbersome; (7) The product cost is high

Method used

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  • Soybean isoflavone extract, its preparation process and pharmaceutical compositions containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1. Conversion is on the influence of three kinds of isoflavone total content

[0028] Isoflavones in soybean mainly include free aglycon and bound glycoside, and aglycone is very small in soybean, mainly in the form of glycoside, including malonyl genistin and malonyl soybean Glycoside, malonyl daidzein, acetyl genistin, acetyl daidzein, acetyl daidzein, genistin, daidzin and daidzein etc. Research reports at home and abroad show that genistin, daidzin and daidzein are the main active ingredients, but there are few research reports on malonyl and acetyl glycosides. In the process research, we found that heating can convert malonyl and acetyl glycosides into their respective glycosides, so we adopted the method of controlling heating conditions during the flocculation heating process to convert all malonyl and acetyl glycosides into their corresponding glycosides , thereby increasing the content of active ingredients.

[0029] Get soybean isoflavone extract ...

Embodiment 2

[0032] Embodiment 2: the impact of washing times on purity

[0033] The total soybean isoflavone precipitation content obtained after flocculation is about 30%. After in-depth research, it is found that the content of soybean isoflavone glycosides in the sediment can be gradually increased by washing with water. The more washing times, the higher the purity of soy isoflavone glycosides.

[0034] Weigh the crude extract of soybean isoflavones obtained after flocculation according to the aforementioned conditions, add 10 times of water to wash each time, centrifuge (3500r / min), and dry the precipitate at 60°C under reduced pressure to measure the content of soybean isoflavones. The results are as follows (Table 2):

[0035] Wash times

[0036] Wash times

[0037] It can be seen from the above experimental results that the three parallel experiments show that the total content of soybean isoflavones after flocculation is about 30%, the total content of soybean ...

Embodiment 3

[0038] Embodiment 3: Preparation scheme 1

[0039] Take 30.4 tons of northeast soybeans and extract soybean oil to obtain about 24 tons of soybean meal. Continuous sampling, 1 ton per hour, 36 tons of 80% edible alcohol, 1.5 tons of alcohol per hour, continuous extraction at 65°C using a ring extractor. After the extract is filtered, it passes continuously through two stages of 60m 2 / L film evaporator after entering 10m 3 The kettle-type vacuum concentration tank is further concentrated to a specific gravity of about 1.20.

[0040] Take about 9kg of flocculant and dissolve it in 15L of deionized water, add it to the kettle-type concentration tank, stir and mix, then add deionized water to adjust the solid content to 30%, stir and mix, heat to 90°C, and keep warm for 30 minutes After cooling down.

[0041] Cool down to below 15°C, use SS-800 centrifuge for separation at 1600 rpm, and centrifuge for 20 minutes to obtain precipitate A, which is stored in refrigeration. The ...

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PUM

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Abstract

The invention relates the soybean isoflavone extractive, comprising 25-74wt% genistin, 4-15wt% soybean glycosides, 3.5wt% chrysophanin. The invention also relates the medicinal composition of soybean isoflavone extractive and preparing method.

Description

technical field [0001] The invention relates to a soybean isoflavone extract, a preparation method thereof and a pharmaceutical composition containing the soybean isoflavone extract. Background technique [0002] Isoflavones exist in a variety of plants and are recognized as an important class of substances in the field of plant extracts. Soy is an important source of isoflavones in particular. Soybean is the mature seed of soybean Glycine max (L.) merr., a leguminous plant, and its medicinal value was first recorded in "Shen Nong's Herbal Classic". Soybean isoflavones are a class of secondary metabolites formed during soybean growth, mainly consisting of 3 aglycones and 9 glycosides formed from them. In soybeans, only a small amount of isoflavones exists in free form, and most of them exist in the form of β-glucoside. In the isoflavone glucoside, some of the C-6 hydroxyl groups on glucose are replaced by malonyl groups, and some of the C-6 hydroxyl groups on glucose are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07A61K36/48
Inventor 廖幼鸣王海燕刘林刘晔赵颖涛王红杨福艳
Owner 北京云威科技有限公司
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