Pollen tube introduction method for flower
A technology for pollen tubes and flowers, which is applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, to achieve the effects of shortening breeding years, wide application value and future, and easy operation.
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Embodiment 1
[0021] The acceptor material is pure white cyclamen, and the donor is: pure white cyclamen, yellow primrose, and plasmid pWM101chsA containing a gene related to controlling flower color--chalcone synthase gene. When the plant selected as the female parent grows to 2.5-3.0 cm, the petals and stamens are removed from the base of the bud, leaving only the pistil covered by the calyx, and immediately bagged and wait for pollination; 1 × SSC solution or Plasmid pWM101chsA dissolved in 1×SSC solution was mixed with pollen of pure white cyclamen and yellow primrose to make a paste, applied on the stigma, bagged separately, and inoculated 1-3 times every other day.
[0022] The plants introduced with exogenous DNA are carefully maintained, and the seeds are harvested in summer and sown in the autumn of that year. Seen from the growth and development of seedlings, the transformed plants generally grew slightly slower, with weaker growth and poorer plant types than the control. After e...
Embodiment 2
[0024] The acceptor material is pure white cyclamen, and the donor is: pure white cyclamen, yellow primrose, and plasmid pWM101chsA containing a gene related to controlling flower color--chalcone synthase gene. When the flower buds of the plants selected as female parents grow to 2.5-3.0 cm, the petals and stamens are removed from the base of the buds, leaving only the pistil covered by the calyx, and immediately bagged and wait for pollination; on the first day, pure white Pollen: cut off the stigma on the second day, mix 1×SSC solution or plasmid pWM101chsA dissolved in 1×SSC solution with yellow primrose pollen to make a paste, apply on the stigma, and bag separately; repeat the third day on the second day way of doing.
[0025] The plants introduced with exogenous DNA are carefully maintained, and the seeds are harvested in summer and sown in the autumn of that year. Seen from the growth and development of seedlings, the transformed plants generally grew slightly slower, ...
Embodiment 3
[0028] The acceptor material of the present invention is a pure white cyclamen variety, and the donor is a rose cyclamen variety, a plasmid pWM101chsA or pBI121chsA containing a gene related to controlling flower color-chalcone synthase gene. When the plant selected as the female parent grows to 2.5-3.0cm, the petals and stamens are removed from the base of the bud, leaving only the pistil covered by the calyx, and immediately bagged and wait for pollination; dissolve in 1×SSC The plasmid pWM101chsA or pBI121chsA of the solution was mixed with the pollen of Cyclamen rose red variety to make a paste, applied on the stigma, bagged separately, and inoculated 1 to 3 times every other day.
[0029] The plants introduced with exogenous DNA are carefully maintained, and the seeds are harvested in summer and sown in the autumn of that year. Seen from the growth and development of seedlings, the transformed plants generally grew slightly slower, with weaker growth and poorer plant type...
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