Pollen tube introduction method for flower

A technology for pollen tubes and flowers, which is applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, to achieve the effects of shortening breeding years, wide application value and future, and easy operation.

Inactive Publication Date: 2006-12-13
天津市园林绿化研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] But so far, the pollen tube channeling method is mostly used in crop breeding, and few people use it in flower transgenic breeding.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The acceptor material is pure white cyclamen, and the donor is: pure white cyclamen, yellow primrose, and plasmid pWM101chsA containing a gene related to controlling flower color--chalcone synthase gene. When the plant selected as the female parent grows to 2.5-3.0 cm, the petals and stamens are removed from the base of the bud, leaving only the pistil covered by the calyx, and immediately bagged and wait for pollination; 1 × SSC solution or Plasmid pWM101chsA dissolved in 1×SSC solution was mixed with pollen of pure white cyclamen and yellow primrose to make a paste, applied on the stigma, bagged separately, and inoculated 1-3 times every other day.

[0022] The plants introduced with exogenous DNA are carefully maintained, and the seeds are harvested in summer and sown in the autumn of that year. Seen from the growth and development of seedlings, the transformed plants generally grew slightly slower, with weaker growth and poorer plant types than the control. After e...

Embodiment 2

[0024] The acceptor material is pure white cyclamen, and the donor is: pure white cyclamen, yellow primrose, and plasmid pWM101chsA containing a gene related to controlling flower color--chalcone synthase gene. When the flower buds of the plants selected as female parents grow to 2.5-3.0 cm, the petals and stamens are removed from the base of the buds, leaving only the pistil covered by the calyx, and immediately bagged and wait for pollination; on the first day, pure white Pollen: cut off the stigma on the second day, mix 1×SSC solution or plasmid pWM101chsA dissolved in 1×SSC solution with yellow primrose pollen to make a paste, apply on the stigma, and bag separately; repeat the third day on the second day way of doing.

[0025] The plants introduced with exogenous DNA are carefully maintained, and the seeds are harvested in summer and sown in the autumn of that year. Seen from the growth and development of seedlings, the transformed plants generally grew slightly slower, ...

Embodiment 3

[0028] The acceptor material of the present invention is a pure white cyclamen variety, and the donor is a rose cyclamen variety, a plasmid pWM101chsA or pBI121chsA containing a gene related to controlling flower color-chalcone synthase gene. When the plant selected as the female parent grows to 2.5-3.0cm, the petals and stamens are removed from the base of the bud, leaving only the pistil covered by the calyx, and immediately bagged and wait for pollination; dissolve in 1×SSC The plasmid pWM101chsA or pBI121chsA of the solution was mixed with the pollen of Cyclamen rose red variety to make a paste, applied on the stigma, bagged separately, and inoculated 1 to 3 times every other day.

[0029] The plants introduced with exogenous DNA are carefully maintained, and the seeds are harvested in summer and sown in the autumn of that year. Seen from the growth and development of seedlings, the transformed plants generally grew slightly slower, with weaker growth and poorer plant type...

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Abstract

The invention discloses a kind of screening method for barley re-cadmium-poison genotype, comprises selecting seed, disinfecting, culturing seedling, transplanting, pre-culturing, cadmium coerion treatment, re-cadmium-poison evaluation; cadmium treat 20 days, detect value of the chlorophyll device for the middle of the functional leave; the decline ratio(%)= 100X(20Umcd treatment detection value - concrete with detection value)/ concrete with detection value ; the integration score of each breed= value of the chlorophyll device declined ratioX0.5+ root volume declined ratioX0.1+root number declined ratioX0.1+ dry weight of underground declined ratioX0.1+stalk high declined ratioX0.1.

Description

technical field [0001] The invention relates to flower breeding technology, in particular to a pollen tube introduction method for flowers using pollen tube channel transgenic technology. Background technique [0002] In the late 1970s, on the basis of distant hybridization, Chinese scholars used the method of mixing pollen homogenate of heterogeneous crops during pollination, and found that there were variations in offspring corresponding to the traits of heterogeneous crops, speculating that foreign DNA may be involved in the fertilization process. Combined with the successful experience of distant hybridization in my country, Zhou Guangyu et al. put forward the hypothesis of hybridization of DNA fragments in distant hybridization from a biochemical point of view, and based on this assumption, designed a method for introducing foreign DNA after self-pollination. Pollen tube channel transgenic technology. Its main principle is that after pollination, exogenous DNA can infil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 付新生赵万苓姜世平
Owner 天津市园林绿化研究所
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