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Investigating method of spore individual number

A detection method and spore technology, which is applied in the field of detection of the number of spore individuals, can solve the problems of inaccurate counting, laborious, unable to record the actual state, etc.

Inactive Publication Date: 2006-11-29
孙庆海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection methods usually used by people are visual inspection under microscope conditions, such as aliquot estimation method, blood cell counter method, etc., which have the defects of time-consuming, laborious, inaccurate counting and inability to record the actual state

Method used

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Examples

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Embodiment Construction

[0006] The specific method is:

[0007] 1. After slightly stirring the spore water body to be tested, extract a unit water body, such as 1 ml, on a slide, or intercept a unit attachment base fragment, place it under the microscope lens, and adjust it to the desired position;

[0008] 2. Use the digital camera on the photomicrograph to take pictures and record the state of the spores, input and store them in the computer;

[0009] 3. Output the paper records through the printer, and then count the individual spores according to the paper records to obtain the number of individual spores per unit water body or attachment base fragment;

[0010] 4. The total amount of individual spores can be obtained by multiplying the number of individual spores per unit water body or attachment base fragment by the total amount of water body or attachment base.

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Abstract

The invention relates to a plant spore number detecting method, especially determination of alga spore number, characterized in using micro imaging to record spore states in unit water body or substrate segment, inputting and storing the spore states into a computer, outputting paper records by printer and then according to the paper records, counting the spores and obtaining the spore number.

Description

technical field [0001] The invention relates to a method for detecting the individual quantity of plant spores, in particular to the determination of the individual quantity of seaweed spores. technical background [0002] Spores are a kind of reproductive or dormant cells produced by plants, which can directly develop into new individuals, such as zoospores (green algae), monospores (red algae), endospores (cyanobacteria), and shells of red algae Spores (Laver), Tetraspores (Glychophyllum), etc. In the breeding process of algae, especially in the artificial large-scale seedling cultivation, the number of individual spores produced by seed algae and successfully attached to the attachment substrate is the main indicator of the quality or success of the seedling cultivation. The detection methods usually used by people are visual inspection under microscope conditions, such as aliquot estimation method, blood cell counter method, etc., which h...

Claims

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Application Information

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IPC IPC(8): G01N15/00C12Q1/02
Inventor 孙庆海
Owner 孙庆海
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