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Method for culturing heterotrophic chlorella with high density

A heterotrophic chlorella, high-density culture technology, applied in the field of high-density culture of heterotrophic chlorella, can solve the problem of low glucose utilization rate of heterotrophic chlorella, to alleviate damage, improve efficiency, reduce production cost effect

Inactive Publication Date: 2006-09-27
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of low glucose utilization rate of heterotrophic chlorella, enhance the tolerance of chlorella to high concentration of glucose, and realize high-density cultivation of heterotrophic chlorella under simplified process conditions

Method used

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  • Method for culturing heterotrophic chlorella with high density
  • Method for culturing heterotrophic chlorella with high density
  • Method for culturing heterotrophic chlorella with high density

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Under the condition of medium glucose concentration of 10g / l, the effect of adding CSL or not on the growth state of heterotrophic algae cells is shown as follows figure 1 shown.

[0024] A glucose concentration of 10 g / l is widely reported as an optimal condition for the growth of heterotrophic Chlorella. After 144 hours of cultivation, without adding CSL, the algal cell density was 3.74g / l; after adding 1‰ of CSL, the cell density was as high as 10.95g / l, which was 293% of the control group. The residual sugar in the culture solution without CSL was as high as 6.49g / l, and the residual sugar in the culture solution added with CSL was only 0.12g / l, and the utilization rate of glucose increased from 35.1% to 98.8%. It indicated that the addition of CSL greatly promoted the growth of heterotrophic Chlorella and fully utilized glucose.

Embodiment 2

[0026] Under the condition of medium glucose concentration of 30g / l, whether to add CSL or not to the influence of the growth state of heterotrophic algae cells, the results show as follows figure 2 shown.

[0027] After 144 hours of cultivation, without adding CSL, the algal cell density was 0.22g / l, compared with the algal cell concentration of 0.17g / l after inoculation, it can be considered that the high concentration of glucose of 30g / l basically inhibited the growth of algae. After adding 1.5‰ of CSL, the cell density reached 18.01g / l, the residual sugar was 0.13g / l, and the utilization rate of glucose was 99.6%. It indicated that the addition of CSL alleviated the damage of high concentration glucose to Chlorella and stimulated the growth of Chlorella.

Embodiment 3

[0029] Under the condition of medium glucose concentration of 50g / l, the effect of adding CSL or not on the growth state of heterotrophic algae cells is shown as follows image 3 shown.

[0030] After 192 hours of cultivation, the growth of algae was inhibited without adding CSL. After adding 2‰ of CSL, the cell density reached 22.97g / l, the residual sugar was 8.40g / l, and the utilization rate of glucose was 83.2%. It shows that with the increase of glucose concentration, the density of harvested cells increases, but the utilization rate of algal cells to glucose decreases, the growth cycle is prolonged, and the efficiency of converting sugar into biomass decreases.

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Abstract

The invention discloses a small ball algae cultivation method with high density and high quality, which comprises the following steps: adding the culture medium with glucose and a little of CSL addictive in the gas culture bottle; proceeding rapid small ball algae culture in the heterotrophy condition. The invention simplifies the producing craft and reduces the cost, which is benefit to cultivate large-scale high-density small ball algae. When the substrate glucose density is 100 g per L, the obtaining biomass of small ball algae is 34.97 g per L.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for rapidly cultivating chlorella under heterotrophic conditions and cultivating heterotrophic chlorella at high density. Background technique [0002] Chlorella is a single-cell green alga of the genus Chlorella of the phylum Chlorella, which has a wide ecological distribution, is easy to cultivate, has a fast growth rate, and has high application value. Chlorella is rich in protein, polysaccharides, lipids, chlorophyll, vitamins, trace elements and some biologically active metabolites, and is widely used in health food, feed, food additives, fine chemicals and raw materials for pharmaceutical preparations. In addition to the normal growth of Chlorella cells using light energy and carbon dioxide under autotrophic conditions, they can also grow and reproduce using organic carbon sources under heterotrophic conditions. The growth rate is faster than that under light conditions,...

Claims

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Application Information

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IPC IPC(8): C12N1/12
Inventor 吴庆余徐瀚
Owner TSINGHUA UNIV
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