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Preparation method of visible rhinopharyngocele cancer model

A technology for nasopharyngeal carcinoma and nasopharyngeal carcinoma, applied in the field of medical models, can solve problems such as difficulty in detecting early primary microtumors, and achieve the effect of improving sensitivity

Inactive Publication Date: 2006-08-30
SOUTHERN MEDICAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

However, relying solely on microscopy and common pathological detection methods is often difficult to detect early primary micro-tumors (this is also the "bottleneck" that prevents surgical treatment of tumor patients in clinical practice), let alone the detection of early metastatic single tumor cells or tiny tumor cell clusters

Method used

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  • Preparation method of visible rhinopharyngocele cancer model
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  • Preparation method of visible rhinopharyngocele cancer model

Examples

Experimental program
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Effect test

Embodiment 1

[0038] 1. Preparation of nasopharyngeal carcinoma model by orthotopic inoculation of EGFP transfected CNE2 nasopharyngeal carcinoma cells in nude mice.

[0039] The first step: prepare EGFP transfection nasopharyngeal carcinoma cells.

[0040] 24 hours before transfection, the nasopharyngeal carcinoma cell line CNE2 was divided into 2 × 10 5 Inoculate in 24-well plates, culture in 1640 medium containing 15% calf serum and no antibiotics, discard the medium when the cells grow to about 80% confluence, wash the cells twice with 1640 medium without serum, and then Add 1ml of serum-free 1640 to continue culturing, and perform cell transfection when the cells grow to a confluence of about 90%. Prepare solutions A, B in microcentrifuge tubes. Solution A: 50 μl OPTI-MEM+5 μllipofectamine2000; Solution B: 50 μl OPTI-MEM+3 μg plasmid pEGFP-N1. Mix A and B liquids and place at room temperature for half an hour, then evenly drop the mixture on the surface of the transfected cells, mix...

Embodiment 2

[0049] 1. Preparation of EGFP-transfected 6-10B nasopharyngeal carcinoma cell model orthotopic inoculation in nude mice.

[0050] The first step: prepare EGFP transfection nasopharyngeal carcinoma cells.

[0051] The transfection method was the same as that in Example 1, and 6-10B cells were transfected with EGFP by cationic liposome method. 6-10B nasopharyngeal carcinoma cells stably transfected with EGFP gene were obtained which were independent of G418.

[0052] The second step: transfection and inoculation of nasopharyngeal carcinoma cells in the nasopharynx of nude mice to establish a nasopharyngeal carcinoma model in situ.

[0053] The method of transfecting 6-10B nasopharyngeal carcinoma cells with EGFP and inoculating them in mice was the same as that in Example 1. EGFP-transfected 6-10B nasopharyngeal carcinoma cells were inoculated into 25 nude mice.

[0054] 2. Observation results of nasopharyngeal carcinoma model.

[0055] Carefully observe the diet and activit...

Embodiment 3

[0059] 1. EGFP transfection of 5-8F nasopharyngeal carcinoma cells orthotopic inoculation of nasopharyngeal carcinoma models in nude mice.

[0060] Step 1: Preparation of EGFP-transfected nasopharyngeal carcinoma cells

[0061] The transfection method is the same as that in Example 1, and 5-8F cells are transfected with EGFP by cationic liposome method. Obtain 5-8F nasopharyngeal carcinoma cells that are not dependent on G418 and stably transfected with EGFP gene.

[0062] The second step: transfection and inoculation of nasopharyngeal carcinoma cells in the nasopharynx of nude mice to establish a nasopharyngeal carcinoma model in situ.

[0063] The method of transfecting 5-8F nasopharyngeal carcinoma cells with EGFP and inoculating them in mice is the same as that in Example 1. EGFP-transfected 5-8F nasopharyngeal carcinoma cells were orthotopically inoculated into 23 nude mice.

[0064] 2. Observation results of nasopharyngeal carcinoma model.

[0065] Carefully observe ...

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Abstract

A process for preparing the visualized nasopharyngeal carcinoma model includes such steps as using green fluorescin gene for the transfection to the cells of nasopharyngeal carcinoma, using the proper needle to take the suspension of the living cancer cells, and in-situ inoculating to the nasopharyngeal position of mouse via mouth.

Description

technical field [0001] The invention relates to the technical field of medical models, in particular to a method for preparing a tumor model, especially a method for preparing a nasopharyngeal carcinoma metastasis model. Background technique [0002] To understand the law of tumor occurrence, development and metastasis, the most important thing is to find it in the early stage of tumor occurrence and metastasis. If it can be observed and detected at the single-cell level or small tumor cell clusters, it will be very important for understanding the occurrence, development and metastasis of tumors. The law of its evolution will be of great significance. Therefore, it is very necessary to study tumors, especially small tumors, to understand and control the occurrence, development and distant metastasis of tumors. However, relying solely on microscopes and common pathological detection methods is often difficult to detect early primary small tumors (this is also the "bottleneck...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
Inventor 姚开泰丁彦青刘腾飞任彩萍
Owner SOUTHERN MEDICAL UNIVERSITY
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