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Method of judging the onset of periodontitis

A technique for periodontitis and periarthritis, which is applied in the fields of disease diagnosis, biochemical equipment and methods, and microbial determination/examination. Difficulty distinguishing, etc.

Inactive Publication Date: 2006-08-02
APPL CELL BIOTECHNOLOGIES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Usually, there is a problem that when the inflammation of the gums is confirmed by inspection, etc., it takes a lot of experience and skill to distinguish gingivitis from periodontitis, and it is difficult to clearly distinguish and diagnose gingivitis from periodontitis
In addition, there is the following problem. The distinction between gingivitis and periodontitis is often judged based on the experience and skills of the dentist, so there are individual differences in the judgment criteria of the dentist.
Furthermore, with regard to the conventional method of measuring various enzymes for the diagnosis of periodontal disease, it is also difficult to clearly distinguish gingivitis from periodontitis, and there are also problems such as complicated operation procedures, low sensitivity, and high technology requirements
[0013] For example, in the invention of Patent Document 1, the degree of inflammation of the periodontal tissue is judged by measuring the increase in the activity of enzyme / peroxidase, because in the case of gingivitis and in the case of periodontitis, the peroxidized Therefore, it is difficult to clearly judge the difference between gingivitis and periodontitis
[0014] In the invention of Patent Document 2, the inflammation or damage of the periodontal tissue is judged by measuring the increase in the concentration of enzyme / ALT, because as above, in the case of gingivitis and in the case of periodontitis, the ALT Enzyme activity increased, although it can predict the progression of periodontal disease symptoms to some extent, it is difficult to clearly distinguish the difference between gingivitis and periodontitis
[0015] Regarding the invention of Patent Document 3, it is difficult to clearly distinguish the difference between gingivitis and periodontitis, although it may be possible to predict the progress of periodontal disease to some extent by using the enzyme released by the pathogenic bacteria of periodontal disease as an index
In addition, since there are individual differences in the correlation between the progression of periodontal disease and bacterial proliferation, it is difficult to clearly distinguish whether each patient has gingivitis or periodontitis even if the measured value of enzymes released by bacteria is the same in multiple patients
[0016] As described above, there is a problem that there is no simple and clear method for distinguishing between gingivitis and periodontitis in the prior art

Method used

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  • Method of judging the onset of periodontitis
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  • Method of judging the onset of periodontitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In Example 1, the gingival crevicular exudate was collected to measure the enzyme activity of γ-GTP to distinguish gingivitis from periodontitis, and the above experiment was carried out according to the following steps. However, the present invention is not limited to the examples.

[0038] First, gingival crevicular exudates were collected from three subjects (patients cured of periodontitis, patients with gingivitis, and patients with periodontitis). The gingival sulcus exudate was collected by inserting a paper point into the gingival sulcus of each subject for 1 minute, and then detecting the gingival sulcus exudate infiltrated into the paper point. Among them, in patients with previously cured periodontitis, the gingival sulcus exudates were collected from two places: a periodontal pocket with a shallow gingival sulcus and a deep periodontal pocket located elsewhere. In addition, in patients with periodontitis, from the part of the gingival sulcus where the curre...

Embodiment 2

[0048] Example 2 is an experiment for confirming the expression of γ-GTP mRNA by preparing gingival crevicular exudates from patients with periodontal disease using the genetic detection method.

[0049] First, RNA was extracted from gingival crevicular exudates of patients with periodontal disease using a kit for RNA extraction / ISOGEN (manufactured by NIPPON GENE Co., Ltd.). Then, target DNA was synthesized and amplified by RT-PCR using an RT-PCR high-Plus-kit (manufactured by TOYOBO). As primers for RT-PCR, 5'-TCCCTTGACCTTCAGGAGAACGAG-3' and 5'-GTGTGGTGCTGTTGTAGATGGTGA-3' were used. As a result, the target NDA was amplified by the PCR method using these primers, and it was thus confirmed that human γ-GTP mRNA was expressed in the gingival crevicular exudate.

[0050] From the above results, it was shown that γ-GTP in the gingival crevicular exudate can be detected even by the genetic detection method.

Embodiment 3

[0052] Example 3 is an experiment showing that γ-GTP participates in the destruction of alveolar bone using experimental periodontal disease model rats, and the steps are as follows.

[0053] Seven-week-old Wistar rats were immobilized under anesthesia, and LPS was brought into contact with the occluded left and right molars of the rats for 1 hour. In addition, after 0, 1, 2, 3, and 7 days, they were euthanized to collect periodontal tissues, fixed in formalin by conventional methods, and made pathological sections. Among them, "LPS" refers to lipopolysaccharide (lipopolysaccharide), which is an important component of the outer membrane of Gram-negative bacteria. As an endotoxin, LPS has various biological activities. In this experiment, LPS was used to sensitize the molars of rats for a certain period of time to experimentally cause periodontitis. As LPS, LPS derived from Escherichia coli was used.

[0054] Next, the pathological sections were first stained with γ-GTP ( fi...

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Abstract

The present invention provides a method of gingivitis and periodontitis which are distingushed simply. The inventor discovered that gamma-GTP (gamma-glutamyl transpeptidase) was scarcely contained in the gingival crevicular fluid of patients with gingivitis, but was contained in the gingival crevicular fluid of patients with periodontitis. Thus, the inventor provides a determination method of development of periodontitis by detecting or measuring gamma-GTP contained in the gingival crevicular fluid. Also, this method can be applied to the determination of the development of peri-implant inflammation.

Description

technical field [0001] The invention relates to a method for judging the onset of periodontitis and a method for judging the onset of peri-implantitis. More specifically, it relates to a method for diagnosing the onset of periodontitis and a method for diagnosing the onset of peri-implantitis characterized by detection or measurement of γ-GTP (γ-glutamyl transpeptidase). Background technique [0002] The term "periodontal tissue" refers to the tissue that is located around the teeth and plays a role in supporting the teeth, and is composed of gingiva, periodontal ligament, cementum, and alveolar bone. Among the periodontal tissue inflammations, the inflammation limited to the gum is called "gingivitis", and the inflammation site exceeds the gum and spreads to the periodontal ligament or the alveolar bone is damaged and destroyed as "periodontitis". [0003] Both gingivitis and periodontitis are caused by inflammation of the periodontal tissue due to the proliferation of bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/573C12Q1/48C12Q1/68C12N15/09G01N33/50
CPCC12Q1/48G01N2333/9108G01N2800/18
Inventor 石塚保行新饭田俊平
Owner APPL CELL BIOTECHNOLOGIES INC
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