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Computerized colorimetric microbial biochemical identification method

A microbial biochemical and identification method technology, applied in the field of biochemical identification of microorganisms through computer colorimetry, can solve the problems of time-consuming, cumbersome identification process, misjudgment, etc., and achieve the effect of reducing human misjudgment

Inactive Publication Date: 2006-08-02
郭新 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The identification process is cumbersome and time-consuming, and it is easy to cause human misjudgment

Method used

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  • Computerized colorimetric microbial biochemical identification method
  • Computerized colorimetric microbial biochemical identification method
  • Computerized colorimetric microbial biochemical identification method

Examples

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Effect test

Embodiment 1

[0016] figure 1 , figure 2 It is a specific embodiment of the present invention. This embodiment is to the identification procedure of bacterium: (1) isolate and purify bacterial sample by conventional method; The order of the cups, put the biochemical cup on the cup holder, add the bacterial suspension to each biochemical identification cup with a pipette according to the amount of 150 μl, and cover the lid; (4) Put it into the incubator, Cultivate at 35°C-37°C for 18-24 hours, and observe the results; (5) Input the biochemical characteristics of the cultured bacteria into the colorimetric template on the computer screen for colorimetric identification through the mouse, and automatically code and retrieve the results after the machine is installed Bacterial names, and then transfer the results to the drug susceptibility system to screen sensitive antibacterial drugs and prescribe the right medicine for patients.

Embodiment 2

[0018] image 3 It is a specific embodiment of the present invention. This embodiment is to the identification program of bacterium:

[0019] (1) Separate and purify the bacterial specimens by conventional methods; (2) Prepare the purified strains into a bacterial suspension with a concentration of 0.5-0.6 McFarland units; (3) According to the order of 15 biochemical cups, the bacterial suspensions Use a pipette to add 150 μl of the liquid into each biochemical identification cup, and cover the lid; (4) put it in an incubator, and cultivate it at 37°C for 24 hours, and observe the results; (5) put the After the cultivation, the biochemical characteristics of the bacteria are identified by inputting the colorimetric template on the computer display screen with the mouse. After the computer is installed, the name of the bacteria is automatically coded and retrieved, and then the results are transferred to the drug sensitivity system to screen sensitive antibacterial drugs and p...

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Abstract

The present invention relates to a microbial biochemical identification method by utilizing computer colorimetry. It is characterized by that the biochemical characteristics of cultured microbe can be undergone the process of colorimetric treatment by means of colorimetric template on the computer display screen, then inputted into computer to make identification.

Description

(1) Technical field [0001] The invention relates to a method for biochemical identification of microorganisms, in particular to a method for biochemical identification of microorganisms by computer colorimetry. (2) Background technology [0002] The traditional manual identification method in my country is to manually interpret the biochemical characteristics of microorganisms; however, many semi-automatic identification instruments in foreign countries need to check each biochemical characteristic one by one and determine the corresponding data of its biochemical reaction to determine its positive (+) and negative (-) results. Enter the "+" and "-" signs into the computer. The identification process is cumbersome and time-consuming, and it is easy to cause human misjudgment. (3) Contents of the invention [0003] In order to make up for the deficiencies of the prior art, the present invention provides a computer colorimetric microbial biochemical identification method whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/27C12Q1/02
Inventor 郭新郭柏均叶宁郭振和
Owner 郭新
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