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Rice aroma gene of paddy rice and constructing process and use of expression vector including the rice aroma gene

A technology of gene expression and rice, applied in the direction of genetic engineering, plant genetic improvement, botany equipment and methods, etc., can solve the problems of no cloned gene controlling rice fragrance, no evidence of functional complementarity, etc.

Inactive Publication Date: 2006-08-02
YANGZHOU UNIV
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Problems solved by technology

This is the latest report on the Mixiang gene that can be seen so far. The author only speculates that this gene may be related to Mixiang, but there is no evidence of the most critical functional complementarity, which means that no gene controlling Mixiang has been cloned so far.

Method used

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Embodiment Construction

[0012] According to the technical method of the invention, the cloning of target genes such as Mixiang can be realized, and the cloned genes can be applied to fields such as breeding.

[0013] 1. Fine mapping of genes: use indica and japonica rice hybrids with different performances in target traits (such as rice fragrance traits), and combine F2, F3, BC1, etc. to separate offspring populations. Target genes were mapped using SSR molecular markers on the rice genome. In the mapped gene region, high-density molecular markers were developed according to the differences in the genome sequence of indica and japonica rice, and the target gene was finely mapped. In this study, a total of 8 segregated offspring populations were assembled, with a total of 2891 individual plants. The region of the rice fragrance gene was determined by positioning, and a high-density molecular marker with an average physical distance of only 30kb was developed in the rice fragrance gene region according...

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Abstract

The present invention relates to rice breeding gene technology in biotechnology field, and is rice aroma gene of paddy rice and the constructing process and use of expression vector including the rice aroma gene. The rice aroma gene of paddy rice has nucleotide of total length of 9066 bases and including 1242 bases in start codon ATG upstream, 5859 bases in the gene region and 1965 bases in the termination codon TAA downstream, coding area comprising 15 exons, and gene sequence as changed betaine aldehyde dehydrogenase coding gene Badh with an 8 base deletion in the 7-th exon. Through cutting the integral rice aroma gene of paddy rice of the positive BAC clone with the limiting cleavage sites HindIII on two sides of the rice aroma gene of paddy rice Badh and cloning to expression vector pCAMBIA1302, the expression vector of rice aroma gene of paddy rice may be obtained and may be used in breeding excellent rice variety.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to rice breeding gene technology. Background technique [0002] Since aroma is one of the important characteristics of high-quality rice, extensive research has been carried out worldwide. The gene controlling rice aroma has been located on rice chromosome 8, within about 386kb of molecular markers RM515 and SSRJ07. By sequencing the genes in this region, Bradbury et al. (2005) discovered a gene with sequence differences between rice fragrant and non-fragrant varieties, which encodes trimethylglycinaldehyde dehydrogenase (Beitaine Aldehyde dehydrogenase) , speculated that it may be related to the synthesis of Mixiang. This is the latest report about Mixiang gene that can be seen so far. The author only speculates that this gene may be related to Mixiang, but there is no evidence of the most critical functional complementation, which means that no gene controlling Mixiang has been clon...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82
Inventor 徐明良陈赛华杨毅史薇薇吴娟
Owner YANGZHOU UNIV
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