Agricultural waste fermenting bacterial prepn and its prepn process
A bacterial strain, the technology of Streptomyces luteus, is applied in the field of bacterial preparation and the preparation of the bacterial preparation, which can solve the problems of insufficient fermentation, incomplete killing of harmful organisms, and low fermentation pile temperature, etc.
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[0043] 1. Preparation of ZJY2 inocula:
[0044] ZJY2 Liquid Shake Flask Medium for Fructus Dimosus ZJY2: 1000ml wort (10°Bx), 18g glucose, natural pH. Add 20 grams of agar as the medium to prepare a solid mother seed slant for inoculation and culture. According to the aseptic method, take a loop of the slant mother seed and inoculate it in a yeast liquid shake flask (60ml medium / 250ml Erlenmeyer flask) medium. , 28℃, rotation speed 180rpm, culture for 18 hours, the number of bacteria reaches 0.5×10 8 More than cfu / ml is fine. The solid fermentation medium of Fructus saccharomyces cerevisiae: with 99% bran and a small amount of inorganic salt MgSO 4 , NaCl 0.05% each, add water according to the material to water ratio 1:1 (w / w) to make the material water content about 60%. Take the liquid bacteria cultured in the shake flask as the strain, and inoculate it evenly in the solid fermentation koji (the koji is wooden, the length, width and height are 60cm, 45cm, and 6cm respectively, a...
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