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The pIFN-gamma gene adjuvant for pig vaccine and its prepn process

A gene adjuvant and vaccine technology, applied in the field of pig vaccines, can solve problems such as swelling, pain, fever, adverse reactions, and allergies, and achieve the effects of simple preparation, low cost, and reduced preparation costs

Active Publication Date: 2006-05-17
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

It acts as a storage and carrier for antigens, slowly releasing antigens to prolong the induction of the humoral immune response of the body, but the disadvantage is that it only induces Th2 humoral immune response, and the antibodies are mainly IgG1 type without TCL reaction
Although oil adjuvant and Freund's complete adjuvant can significantly improve the immune response, adverse reactions often occur in practical applications, such as swelling at the injection site, pain, fever, or allergies, etc., and are limited to laboratory animal experiments
206 adjuvant is a high-efficiency animal vaccine oil adjuvant that is currently commercialized. It has been widely used in animal vaccines. Practice has proved that it is reliable, effective and safe, but it needs to be imported and the price is high

Method used

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  • The pIFN-gamma gene adjuvant for pig vaccine and its prepn process
  • The pIFN-gamma gene adjuvant for pig vaccine and its prepn process
  • The pIFN-gamma gene adjuvant for pig vaccine and its prepn process

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preparation example Construction

[0022] The preparation method of adjuvant of the present invention is described in detail as follows:

[0023] (1) Isolate mononuclear cells from porcine peripheral blood or lymph nodes, stimulate and culture them with inducers, clone the target cytokine gene by RT-PCR, and analyze the sequence homology, genetic evolution relationship, and molecular structure and function prediction , a candidate gene as a gene adjuvant;

[0024] (2) Using genetic engineering recombination technology, selecting appropriate expression primers containing restriction sites to clone the target cytokine gene into the animal cell expression plasmid vector;

[0025] (3) transforming prokaryotic host bacteria with the recombinant plasmid vector obtained in step (2), selecting positive clones, and performing amplification culture after sequencing and identification; and

[0026] (4) A large amount of recombinant plasmid DNA of the target gene is extracted and purified.

[0027] Wherein the plasmid ca...

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Abstract

The present invention discloses one kind of adjuvant for pig vaccine and its preparation process and constituted pig vaccine. The gene adjuvant for pig vaccine is adjuvant including pig gamma-interferon gene (pIFN-gamma), or recombinant plasmid pcDNA-pIFN-gamma of animal cell expression plasmid pcDNA3.1 and pig gamma-interferon gene (pIFN-gamma), or the combination of pcDNA-pIFN-gamma and No. 206 adjuvant. The pig vaccine is composition of pigí»s cysticercus-resisting vaccine composition; pigí»s deactivated foot-and-mouth disease virus vaccine and the gene adjuvant. The gene adjuvant of the present invention is prepared through gene cloning, recombination, recombinant plasmid proliferation, extraction and purification and other processes.

Description

technical field [0001] The invention relates to an adjuvant used in porcine vaccines, a preparation method thereof, and a porcine vaccine composed of the adjuvant of the invention. Background technique [0002] Since the 1920s, many substances have been tried as immune adjuvants, but only aluminum colloidal adjuvants have been licensed for human vaccines and are widely used in pig vaccines. It acts as a storage and carrier of antigens, and slowly releases antigens to prolong the induction of humoral immune responses in the body. However, the disadvantage is that it only induces Th2 humoral immune responses, and the antibodies are mainly IgG1, without TCL responses. Although oil adjuvant and Freund's complete adjuvant can significantly improve the immune response, adverse reactions often occur in practical applications, such as swelling at the injection site, pain, fever, or allergies, etc., and are limited to laboratory animal experiments. 206 adjuvant is a high-efficiency ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K9/107A61K39/002A61K39/135A61K48/00A61P31/12A61P33/02C12N15/09
Inventor 景志忠才学鹏窦永喜蒙学莲王佩雅陈国华罗启慧袁改玲侯俊玲骆学农
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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