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Method for producing C. ophioglossouides using liquid submerged culture

A technology of liquid submerged fermentation and Cordyceps fungus, which is applied in the field of microbial engineering, to achieve the effect of rich medium material, reasonable nutrient distribution ratio, and reduction of polluting bacteria

Inactive Publication Date: 2006-02-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the scarcity of wild resources and the deterioration of the growth environment, the existing natural resources of this fungus can no longer meet people's needs. It is very necessary to use modern biotechnology to save this precious traditional Chinese medicine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the preparation of seed culture medium

[0022] Weigh 40 grams of sucrose, 20 grams of yeast powder (paste), 30 grams of peptone, MgSO 4 ·7H 2 O 0.2 g, KH 2 PO 4 0.2 g, mix and dissolve each component, add water to make up to 1000 ml, and obtain after sterilization.

Embodiment 2

[0023] Embodiment 2: the preparation of protein hydrolyzate

[0024] Method 1: Take fresh raw materials such as soybean powder, shrimp powder, silkworm chrysalis powder, etc., and dry them in an oven at 60°C for 6 hours, then put them in an oven at 80°C for 17 hours, weigh 100 grams of dry powder, add 6 grams of quicklime, water 640 ml, pressurized and hydrolyzed at 0.15Mpa for 2 hours, filtered, and the filtrate was obtained.

[0025] Method 2: Take fresh raw materials such as soybean powder, shrimp powder, silkworm chrysalis powder, etc., and dry them in an oven at 60°C for 6 hours, then put them in an oven at 80°C for 17 hours, weigh 100 grams of dry powder, and add 6mol / l hydrochloric acid 600 ml, hydrolyzed in a boiling water bath for 30 minutes, filtered and collected the filtrate.

Embodiment 3

[0026] Embodiment 3: the preparation of liquid submerged aerated fermentation medium

[0027] Take by weighing 50 grams of sucrose, 20 grams of yeast powder (paste), 40 milliliters of protein hydrolyzate prepared by Example 2, MgSO 4 ·7H 2 O 0.5 g, KH 2 PO 4 0.5 grams. Mix the above ingredients and add water to 1000 ml, add 0.6 ml of foam diol as an antifoaming agent, adjust the pH to 5.6 with hydrochloric acid, and sterilize it to become the fermentation medium.

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PUM

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Abstract

The invention discloses a process for producing Cordyceps ophioglossoides through liquid submerged culture, which consists of using Cordyceps ophioglossoides obtained by artificial isolated culture as starting bacteria, carrying out oblique plane culture, bottle shaking seed culture and fermentation tank culture, the dry weight of mycelium in the fermentation liquor can reach 2-3%. The liquid seed culture medium and fermentation culture medium comprise sucrose, yeast powder, peptone, magnesium salt, potassium salt, cane sugar, proteolysis liquid.

Description

technical field [0001] The invention relates to the technical field of microbial engineering, in particular to a liquid submerged fermentation culture process of Cordyceps ophioglossoides. Background technique [0002] Cordyceps macrocystis is a precious Chinese herbal medicine, which is widely used by folks in southern my country and has a long history. The Cordyceps fruiting body has high medicinal value in the treatment of gynecological diseases, and is mainly used to treat irregular menstruation, dysmenorrhea, amenorrhea and other diseases, and also has a significant effect on postpartum hemorrhage and promoting postpartum uterine recovery. Due to the scarcity of wild resources and the deterioration of the growth environment, the existing natural resources of this fungus can no longer meet people's needs. It is very necessary to use modern biotechnology to save this precious traditional Chinese medicine. [0003] The modern fermentation technology developed in the middl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 李永泉郑静
Owner ZHEJIANG UNIV
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