Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of improving lactic acid bacteria freeze drying activity using preculturing mode

A lactic acid bacteria and pre-cultivation technology, applied in the field of lactic acid bacteria live bacteria preparation and yogurt freeze-dried starter, to achieve the effects of improving bacterial activity, shortening curdling time, and shortening curdling time

Inactive Publication Date: 2006-01-25
JIANGNAN UNIV
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention refers to this treatment method as pre-cultivation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The strains were Lactobacillus delbrueckii subsp. Bulgaricus (Lactobacillus delbrueckii subsp. Bulgaricus) CTCC6047 and Streptococcus thermophilus (Streptococcus thermophilus) CTCC6038 in a ratio of 1:1 and cultured in the fermentation medium with 2%. The conventional method is through enrichment culture, and the bacteria are collected after centrifugation, added with the above protective agent to suspend, placed at 4°C for 30 minutes and then pre-cultured. Select 20°C, 30°C, 42°C, 55°C for the pre-cultivation temperature and 1 hour, 2 hours, and 3 hours for the pre-cultivation time, and conduct primary selection to investigate the changes in pH and bacterial count during the culture process; investigate the pre-culture The survival rate and clotting time of the treated cells after freeze-drying. The results show that: at the growth temperature of 20°C, the indicators of the bacteria are very close, and the pH and the number of bacteria have no change. With the extensio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method to improve lactic acid bacteria freeze-dry activity that belongs to acidophilus milk freeze-dry ferment and lactic acid bacteria active fungus technology field. It mixes the lactic acid bacteria body before freeze-dry and protection agent, lays at 4 degree centigrade for 30 minutes to balance, under a certain temperature, after a certain time of pre-cultivating, then freezing, drying and storing. The method could improve the freeze-dry activity of lactic acid bacteria.

Description

technical field [0001] The invention discloses a method for improving the freeze-dried activity of lactic acid bacteria by means of pre-cultivation, which belongs to the technical field of yogurt freeze-dried starter and live lactic acid bacteria preparations. Background technique [0002] Due to the problems of cost and industrialization, the most commonly used method for cell collection is centrifugation, that is, the centrifugal force of the centrifuge is used to deposit the cells. The purpose is to separate the cells from the culture medium and metabolites, and to minimize For the damage of bacteria, this method is simple, fast, and has a large amount of treatment, which is suitable for commercial production. After the bacteria are enriched and cultured, collected by centrifugation and suspended with a protective agent, the general measure is to quickly pre-freeze at a low temperature, and freeze-dry after a certain period of time. From the step of suspending the bacter...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/225C12R1/46
Inventor 赵建新陈卫田丰伟张灏潘巧黄文利
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com