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Primary structure of snake origin deintegration element mutant anti human platelet aggregation medicine health beneficial protein

A platelet aggregation and disintegrin technology, applied in peptide sources, blood diseases, drug combinations, etc., can solve problems such as 15 amino acid differences, and achieve the effect of inhibiting angiogenesis

Inactive Publication Date: 2006-01-25
赵宝昌
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AI Technical Summary

Problems solved by technology

However, the amino acid sequence homology between Barbourin and Aidingbit is only 79%, that is, there are 15 amino acid differences

Method used

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Embodiment Construction

[0022] The cloning of Yikang for the prevention and treatment of human thrombosis, including site-directed mutation, is carried out by means of molecular biology. The nucleotide sequence of Yikang contains 222 base pairs, and the nucleotide sequence is:

[0023] 1 GAGGCCGGAG AAGAATGTGA CTGTGGCTCT CCTGGAAATC CGAGCTGTGA

[0024] 51 TGCTGCAACC TGTAAACTGA GACAAGGAGC ACAGTGTGCA GAAGGACTGT

[0025] 101 GTTGTGACCA GTGCAGATTT ATGAAAAAAG GAACAGTATG CCGGATAGCA

[0026] 151 AAG(A)GGTGATG ACATGGATGA TTACTGCAAT GGCATATCTG CTGGCTGTCC

[0027] 201 CAGAAATCCC TTCCATGCCT AG

[0028] Protein Yikang is composed of 73 amino acids, and its amino acid sequence is as follows:

[0029] EAGEECDCGSPGNPCCDAATCKLRQGAQCAEGLCCDQCRFMKKGTVCRIA

[0030] KGDDMDDYCN GISAGCPRNP FHA

[0031] Using the wild-type gene cloning vector pPET23b-Ad preserved in our laboratory as a template, the steps of point mutation are:

[0032] 1. Design primers: First, design upstream and downstream primers including Hind II...

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Abstract

The invention relates to the primary structure of Yikang protein, a diseased snakes disintegrins mutant anti-human platelet aggregation medicament, which concerns the amino acid residue sequence and the corresponding nucleotide sequence of the disintegrins mutant protein from recombinant agkistrodotoxin of cobra-venom, and the actions for inhibiting human platelet aggregation, preventing and treating thrombus, wherein the protein of low molecular weight consisting of 73 amino acid residues contains 12 cysteine residues and form 6 disulfide bonds. The beneficial effects of the invention include, the action for inhibiting platelet aggregation is achieved, and the suppression to blood vessel neogenesis is avoided.

Description

technical field [0001] The invention belongs to the field of biotechnology. It involves the amino acid residue sequence and corresponding nucleotide sequence of the disintegrin mutant protein of the recombined Agkistrodon halys venom, and its antithrombotic effect of inhibiting platelet aggregation, especially the amino acid of the snake-derived disintegrin mutant Yikang protein Residue sequences and their corresponding nucleotide sequences. Background technique [0002] High platelet aggregation is an indicator of blood hypercoagulation. Inhibiting human platelet aggregation can effectively prevent and treat thrombosis caused by platelet aggregation, and is used for preventing and treating heart and brain ischemic diseases and peripheral thromboembolic diseases. It has been believed that certain snake venoms contain factors that inhibit platelet aggregation, but they have not been able to obtain effective ingredients. At present, there are mainly four types of drugs for ...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12A61P7/02
Inventor 赵宝昌
Owner 赵宝昌
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