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Momordica charantic leaf insect-resisting extract, and its preparation and use

A technology of extracts and leaves, applied in the field of new agricultural insecticides, to achieve the effect of simple preparation method, convenient use, and good stability

Inactive Publication Date: 2005-12-21
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no research report on the development of biological insecticides using bitter gourd leaves as raw materials

Method used

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  • Momordica charantic leaf insect-resisting extract, and its preparation and use
  • Momordica charantic leaf insect-resisting extract, and its preparation and use
  • Momordica charantic leaf insect-resisting extract, and its preparation and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The method for preparing the anti-insect extract of bitter gourd leaves by using the waste—the leaves of bitter gourd as a resource comprises the following steps:

[0028] Use diethyl ether as the extraction solvent, take 50g of fresh bitter gourd leaves, 50ml of diethyl ether (≥99%) in the ratio of 1g:1ml, grind under the conditions of pH 7 and temperature 0°C, extract by standing for 0.5h, and use gyration Oscillate at 20r / min for 8-10h to filter, then dilute to 100ml with natural water as stock solution for later use.

[0029] ·Performance testing:

[0030] The anti-insect extract prepared above was taken for detection. The two-dimensional electrophoresis of the protein contained in the anti-insect extract of bitter gourd leaves using ether as the extraction solvent is as follows: figure 1 shown. After the bitter gourd leaves were extracted with ether, 2D-SDS-PAGE was used for protein detection. Between PI 5.0-7.0, seven protein spots with molecular weights of 26K...

Embodiment 2

[0038] Acetone (≥99%) was used as the extraction solvent and shaken for 10 h, and the others were the same as in Example 1. After the leaves of bitter gourd were extracted with acetone, 2D-SDS-PAGE was used for protein detection. Between PI 6.5-7.5, three protein spots with molecular weights of 31KD, 32KD, and 33KD were detected, such as figure 2 indicated by the middle arrow. The above shows that the extract obtained in this example contains proteins with molecular weights of 31KD, 32KD, and 33KD, and the extract is a light green water-soluble liquid. Others are with embodiment 1.

[0039] Application Lab 5-6:

[0040] The bitter melon leaf acetone anti-insect extract stoste prepared in Example 2 is diluted with water so that its concentration is 50%

[0041] (i.e. take 50ml of bitter melon leaf acetone anti-insect extract stock solution, add 50ml of water), smear it on the front of cabbage leaves, dry and raise test insects. The second instar larvae of Plutella xylostel...

Embodiment 3

[0047] Use deionized water as the extraction solvent, grind at pH 6.8 and 4°C, and the others are the same as in Example 1. After the bitter gourd leaves were extracted with deionized water, 2D-SDS-PAGE was used for protein detection. Between PI 6.5-7.5, six protein spots with molecular weights of 22KD, 23KD, 24KD, 25KD, 28KD and 35KD were detected, such as image 3 indicated by the middle arrow. The above shows that the extract obtained in this example contains proteins with molecular weights of 22KD, 23KD, 24KD, 25KD, 28KD and 35KD. The extract is a light green water-soluble liquid.

[0048] Applied Labs 9-10:

[0049] Dilute the bitter gourd leaf deionized water insect-resistant extract stock solution prepared in Example 3 with water to make the concentration 50% (that is, take 50ml of bitter gourd leaf deionized water insect-resistant extract stock solution and add 50ml of water), and smear it on the front of cabbage leaves , and reared the test insects after drying. T...

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Abstract

The present invention relates to a bitter melon leaf insect-resisting extract, its preparation method and application. It is characterized by that it utilizes bitter melon leaf as raw material, and uses deionized water, ethyl ether or acetone as extraction solvent, and adopts the processes of grinding, standing still, oscillation, filtering and constant volume extraction so as to obtain the green liquor containing protein. The ratio of bitter melon leaf and solvent is 1g / 1ml. Said product can obtain good insect-resisting and insect-killing effect.

Description

Technical field: [0001] The invention belongs to a new type of insect-resistant agent for agriculture, and relates to an insect-resistant extract from bitter gourd waste leaves and its preparation and application. In particular, it relates to a water-soluble insect-resistant extract of bitter gourd leaves using deionized water, ether or acetone as an extraction solvent, its preparation and its application in insect resistance and insecticide. Background technique: [0002] Insect pests reduce the yield of crops in a large amount, so it is of great significance to develop new pollution-free insecticides. Although the extensive use of chemical pesticides today can control the occurrence and development of agricultural pests in a timely manner, it has brought many disadvantages, such as the perennial use of chemical pesticides, which makes pests resistant to pesticides, and the residue of pesticides makes the pollution of farmland and agricultural products more and more serious...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/08A01P7/00
Inventor 陈宏李鹏飞徐秋曼王修鲁王振英
Owner TIANJIN NORMAL UNIVERSITY
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