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Method of breeding in vitro of small tuber for dioscorea nipponica Makino

A technology for piercing the dragon yam and micro tubers, which is applied in the field of in vitro tissue culture and micro tuber propagation, can solve problems such as difficulty in solving large-scale production needs, consumption of large tubers, poor seed fullness, etc. The effect of area promotion, simple method and easy to master

Inactive Publication Date: 2005-11-09
NORTHEAST NORMAL UNIVERSITY
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Problems solved by technology

[0003] Seed propagation is difficult to meet the needs of large-scale production due to poor seed plumpness and high vacancy rate; conventional tuber propagation not only consumes a large amount of tubers, but also has a low reproduction coefficient, difficult rooting, limited proliferation speed and quantity, and is difficult to large-scale production. to promote
Whether it is a wild variety or a cultivated variety, the growth cycle is generally 3-5 years, and the growth rate of the tubers is slow. At the same time, it is easily affected by the environment, seasons, diseases and insect pests, etc., and most of the medicinal yam tubers are irregular, which is not conducive to harvesting.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Material preparation and inoculation

[0017] Cut the newly issued tender stems from the vigorously growing Dioscorea chinensis plants, wash them with tap water for 2 hours, and then wash them with distilled water twice; then soak them in 70% ethanol for about 40 seconds on the ultra-clean workbench, and then with 0.1% HgCl 2 Sterilize for 4 minutes, wash with sterile water 6 times, dry the water with sterile filter paper, cut into about 0.5 cm stem segments (each segment has an axillary bud), and stand upright on the MS medium.

[0018] 2. Induction and Proliferation

[0019] The explants inoculated on the MS medium, after 2 to 3 weeks, 1 to 2 small buds will grow from the original axillary buds, and later, the newly grown buds will grow to 2 to 3 small seedlings of axillary buds.

[0020] 3. Rooting

[0021] When the seedlings on the MS medium grow to 3 to 4 cm (about 3 to 4 leaves) and have a small amount of fibrous roots, they are transferred to 1 / 2 MS medium,...

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Abstract

An external microtuber reproduction method for Dioscorea nipponica includes such steps as using the tender stem segment of Dioscorea nipponica, or the stem segment with bud or small tube with bud of its test-tube plantlet as explant, preparing culture medium, inducing test-tube plant, special treating to generate microtuber, and outdoor culture. The method for outdoor culture is also disclosed. Its advantages are short period, high survival rate and no need of ground.

Description

technical field [0001] The invention belongs to plant cultivation and reproduction technology, and in particular relates to in vitro tissue culture and microtuber propagation method of medicinal plant Dioscorea nipponica Makino. Background technique [0002] Dioscorea chinensis is one of the important varieties of traditional Chinese medicinal materials commonly used in my country. Its rhizome is used as medicine, and its main active ingredient is total saponins of dioscin. It is one of the important sources of diosgenin in hormones and contraceptives. Due to the increasing market demand and decreasing wild resources year by year, Dioscorea chinensis has become an endangered and scarce Chinese medicinal material. [0003] Seed propagation is difficult to meet the needs of large-scale production due to poor seed plumpness and high vacancy rate; conventional tuber propagation not only consumes a large amount of tubers, but also has low reproduction coefficient, difficult rooti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王丽陈凤清
Owner NORTHEAST NORMAL UNIVERSITY
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