Method for fabricating section of biologic tissue

Abstract

The invention is the biological tissue slice making method, which comprises the following processes: A. flatten the tissue sample and fix on the vertical plate of the inverse T-shape non-metal bracket, and fix the base plate of the bracket on the carrying platform and make the vertical plate of the bracket in front of the knifeware and parallel the platform of the bracket and the moving direction of the carrying platform; B. the slicing machine simultaneously slices the tissue samples and the vertical plate of the non-mental bracket. The invention makes the slicing face vertical to the interface of each tissue sample layer, and every interface is flat and straight.

Description

technical field [0001] The invention relates to a method for making biological tissue slices. Background technique [0002] When the existing biological tissue slices are made, the biological tissue specimens are fixed on the stage of the microtome. The fixing methods mainly include low-temperature freezing fixation or paraffin embedding; The plane on which the surface of the object stage is located is basically parallel, and the stage will automatically step a short distance after each round of back and forth motion, and the direction of the stepping motion is perpendicular to the direction of the back and forth motion, thus driving the tissue specimen to move back and forth relative to the stationary tool and stepping motion, the tissue specimen is cut into slices step by step. This method is suitable for the situation where the flatness of the interface between the obtained slices is not high, such as in the pathological diagnosis in the hospital, because the pathologica...

AI Technical Summary

Problems solved by technology

[0003] However, when studying flat and layered tissue specimens (such as skin or mucous membranes) in medicine, it is often desired that the cut plane be perpendicular to the interface between the various layers of the specimen, so that the interface between the various layers of the obtained slice is straight, especially It is hoped that the target cells or target tissue layers in the slice can be easily and quickly dug out under the microscope. In this case, the shortcomings of the traditional biological tissue slice production method will appear, because: 1. If the tissue specimen is flattened And tiled on the stage, the flat tissue specimen stretching direction will be basically parallel to the direction of the stage’s back and forth movement, the obtained section will be basically parallel to the interface between the layers of the tissue sample, and the obtained section will be in the Theoretically, it is likely to be only a certain layer of the tissue sample, and the ideal section perpendicular to the interface between the layers of the tissue sample cannot be obtained; The boundary between layers of tissue will be wrinkled and not straight, and it is not conducive to easy and straightforward digging out of the target cells or target tissue layers in the slice under the microscope

Images